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Effect of targeting NOS2 on gene expression of triple-negative breast cancer (metaplastic) SUM159 cell lines

ABSTRACT: Effect of targeting NOS2 on gene expression of triple-negative breast cancer (metaplastic) SUM159 cell lines

PROVIDER: PRJNA981564 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24878793_1.fastq.gz	Fastqsanger.gz
	SRR24878793_2.fastq.gz	Fastqsanger.gz
	SRR24878794_1.fastq.gz	Fastqsanger.gz
	SRR24878794_2.fastq.gz	Fastqsanger.gz
	SRR24878795_1.fastq.gz	Fastqsanger.gz

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Effect of targeting NOS2 on gene expression of triple-negative breast cancer (metaplastic) SUM159 cell lines

Project description:To investigate the role of iNOS in tumor cell morphology and gene expression, we utilized CRISPR-Cas9 gene editing (NOS2 Double Nickase Plasmid, Santa Cruz) to knockout NOS2 in SUM159 cells. After puromycin selection, resistant clones were selected by immunoblotting for iNOS expression and nitrite/nitrate colorimetric assay. We then performed gene expression profiling analysis using data obtained from RNA-seq of parental SUM159 cells compared to NOS2KO SUM159 cells.

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