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Caspase-mediated processing of TRBP regulates apoptosis during viral infection

ABSTRACT: Caspase-mediated processing of TRBP regulates apoptosis during viral infection

PROVIDER: PRJNA982032 | ENA |

REPOSITORIES: ENA

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Caspase-mediated processing of TRBP regulates apoptosis during viral infection

Project description:RNA silencing is a post-transcriptional gene-silencing mechanism mediated by microRNAs (miRNAs). However, the regulatory mechanism of RNA silencing during viral infection is unclear. TAR RNA-binding protein (TRBP) is an enhancer of RNA silencing that induces miRNA maturation by interacting with the ribonuclease Dicer. TRBP interacts with a virus sensor protein, laboratory of genetics and physiology 2 (LGP2), in the early stage of viral infection of human cells. Next, it induces apoptosis by inhibiting the maturation of miRNAs, thereby upregulating the expression of apoptosis regulatory genes. In this study, we show that TRBP undergoes a functional conversion in the late stage of viral infection. Viral infection resulted in the activation of caspases that proteolytically processed TRBP into two fragments. The N-terminal fragment did not interact with Dicer but interacted with type I interferon (IFN) signaling modulators, such as protein kinase R (PKR) and LGP2, and induced ER stress. The end results were irreversible apoptosis and suppression of IFN signaling. Our results demonstrate that the processing of TRBP enhances apoptosis, reducing IFN signaling during viral infection.

2024-04-19 | GSE234549 | GEO

Caspase-mediated processing of TRBP regulates apoptosis during viral infection

2024-04-19 | GSE234547 | GEO

Caspase-mediated processing of TRBP regulates apoptosis during viral infection

Project description:Caspase-mediated processing of TRBP regulates apoptosis during viral infection

| PRJNA982029 | ENA

Caspase-mediated processing of TRBP regulates apoptosis during viral infection

Project description:Caspase-mediated processing of TRBP regulates apoptosis during viral infection

| PRJNA982025 | ENA

Caspase-mediated processing of TRBP regulates apoptosis during viral infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-19 | GSE234550 | GEO

Homo sapiens strain:HeLa

Project description:Caspase-mediated processing of TRBP regulates apoptosis during viral infection

| PRJDB17739 | ENA

LGP2 virus sensor enhances apoptosis by upregulating apoptosis-regulatory genes through TRBP-bound miRNAs during viral infection

Project description:LGP2 virus sensor enhances apoptosis by upregulating apoptosis-regulatory genes through TRBP-bound miRNAs during viral infection

| PRJNA559265 | ENA

Deletion of human tarbp2 reveals cellular microRNA targets and cell cycle function of TRBP

Project description:TRBP has two known functions as Dicer co-factor and PKR inhibitor. However, the role of TRBP in miRNA biogenesis is controversial and its regulation of PKR in mitosis remains unexplored. Here, we generate TRBP KO HeLa cells and find that TRBP depletion alters Dicer processing sites of a subset of miRNAs, but does not affect Dicer stability, miRNA abundance, or Argonaute loading. By generating PACT, another Dicer interactor, and TRBP/PACT double-KO cells, we further show that TRBP and PACT do not functionally compensate each other and that only TRBP contributes to Dicer processing. We also report that TRBP is hyperphosphorylated by JNK in M phase when PKR is activated by cellular dsRNAs. Hyperphosphorylation potentiates the inhibitory activity of TRBP on PKR, suppressing PKR in M-G1 transition. By generating the first human TRBP KO, our study clarifies the role of TRBP and unveils negative feedback regulation of PKR through TRBP phosphorylation.

2014-09-16 | GSE61458 | GEO

Deletion of human tarbp2 reveals cellular microRNA targets and cell cycle function of TRBP

2014-09-16 | E-GEOD-61458 | biostudies-arrayexpress

Effects of Trbp deletion on gene expession in mice hearts

Project description:We generated the cardiac-specific knockout of Trbp (Trbp-cKO) in mice. We profiled the transcriptome in both wild-type and Trbp-cKO hearts, and found numerous genes were deregulated by deletion of Trbp. We also profiled miRNA expression both wild-type and Trbp-cKO hearts, and found expression of a subset of miRNA species was altered in Trbp-cKO hearts.

2015-04-27 | GSE67658 | GEO

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