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RNA sequencing of LT-HSCs in the bone marrow from WT and Trim47-/- mice untreated or treated with 5-FU for 10 days

ABSTRACT: RNA sequencing of LT-HSCs in the bone marrow from WT and Trim47-/- mice untreated or treated with 5-FU for 10 days

PROVIDER: PRJNA992402 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25183856_1.fastq.gz	Fastqsanger.gz
	SRR25183856_2.fastq.gz	Fastqsanger.gz
	SRR25183857_1.fastq.gz	Fastqsanger.gz
	SRR25183857_2.fastq.gz	Fastqsanger.gz
	SRR25183858_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of LT-HSCs in the bone marrow from WT and Trim47-/- mice untreated or treated with 5-FU for 10 days

Project description:LT-HSCs in the bone marrow of WT and Trim47-/- mice untreated or treated with 5-FU for 10 days were isolated to explore the differentially expressed genes by RNA-seq.

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Project description:Analysis of gene-expression alterations in primitive LT-HSCs as early as 2 days post sex steroid ablation. This study aimed to determine potential intrinsic changes occuring in the LT-HSC population associated with sex steroid ablation (SSA) at a timepoint that preceeded the earliest reported functional hematopoietic enhancements occuring at day 7 post SSA. These results are vital in providing insight into the role of LT-HSCs in initiating SSA-mediated immune regeneration.

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Gene expression profiling of LT-HSCs and ST-HSCs conditionally expressing MLL-ENL

Project description:We have developed a new conditional transgenic mouse showing that MLL-ENL, at an endogenous-like expression level, induces leukemic transformation selectively in LT-HSCs. To investigate the molecular mechanism of leukemic transformation in LT-HSCs conditionally expressing MLL-ENL, we preliminarily performed comprehensive gene expression profiling of CreER-transduced LT-HSCs and ST-HSCs using cDNA microarray analysis. For initial screening of candidate genes invloved in the leukemic transformation, total RNA was extracted from colony-forming cells derived from LT-HSCs and ST-HSCs transduced with CreER or mock. Four samples were analyzed, and CreER-transduced LT/ST-HSC-derived cells were compared with mock-transduced LT/ST-HSC-derived cells, while CreER/mock-transduced LT-HSC-derived cells were compared with CreER/mock-transduced ST-HSC-derived cells.

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Project description:We performed tagmentation-based whole-genome bisulfite sequencing on young and aged LT-HSCs.

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miRNA profiling data of mouse bone marrow samples four days post 5-FU treatment versus untreated controls

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Ex vivo cultured mouse LT-HSCs RNA-seq

Project description:Mouse LT-HSCs were co-cultured with polymer nanospheres for ex vivo expansion

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