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V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

ABSTRACT: V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

PROVIDER: PRJNA99537 | ENA |

REPOSITORIES: ENA

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V. vinifera 'Cabernet sauvignon' and V. aestivalis 'Norton' innoculated with Erysiphe necator conidiospores

Project description:we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera ‘Cabernet sauvignon’ and Vitis aestivalis ‘Norton’ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Keywords: time course

2007-11-29 | GSE6404 | GEO

Transcription profiling of V. vinifera Cabernet sauvignon and V. aestivalis Norton innoculated with Erysiphe necator conidiospores

Project description:we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera âCabernet sauvignonâ and Vitis aestivalis âNortonâ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Experiment Overall Design: sixty plants were grown in each of two identical growth chamber labeled as Inoculation and Mock-inoculation under same conditions. Pairs of vines of same size were labeled, one with an âIâ, the other with a âMâ prefix for âinoculatedâ and âmockâ. The vines in each pair received the same ID number. This resulted in balanced groups for inoculated and mock-inoculated vines. âIâ and âMâ vines of a pair were placed in corresponding locations on the bench of the two growth chambers. The locations of the vines were determined by randomizing their ID numbers. Thus, the âIâ and âMâ groups were balanced according to their size and to their locations within the growth chamber. All vines were positioned at the same height from the light source. The locations of the individual plants within the growth chamber were recorded. At a time point, 10 randomly selected pairs of plants were sampled. (For example, at 8 hours post-inoculation, vines I-3, I-17, I -23, ..etc., and vines M-3, M-17, M-23, ...etc. were sampled from the âIâ and the âMâ growth chambers, respectively). The 10 corresponding leaves were combined into a single sample. At the next time point, another set of randomly selected 10 pairs were sampled from among the remaining plants, and so on. This random selection was independent from the random selection implemented to determine the location of the vines in the growth chambers

2008-06-14 | E-GEOD-6404 | biostudies-arrayexpress

short term abiotic stress Cabernet Sauvignon

Project description:Cabernet Sauvignon plants grown in a hydroponic drip system were treat with 120 mM salt (10:1 NaCl:CaCl), Polyethylene Glycol, cold (5 C) or unstressed. Shoots with leaves were collected at 0, 1, 4, and 8 hours for all treatments, and at 24 hours for all except cold. ****[PLEXdb (http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Elizabeth Tattersall. The equivalent experiment is VV1 at PLEXdb.]

2011-08-23 | GSE31594 | GEO

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Project description:Vitis vinifera bud (Cabernet Sauvignon) RNA sequencing (Transcriptome study)

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Project description:Cabernet Sauvignon Iso-Seq transcriptome reconstruction and genome annotation

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Erysiphe necator

Project description:Erysiphe necator overview

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