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Runx3 function in splenic NK cells (IL-2 or IL-15)

ABSTRACT: NK cells are innate immune cells that recognize and kill foreign, virally-infected and tumor cells without the need for prior immunization. NK expansion following viral infection is IL-2 or IL-15-dependent. To identify Runx3 responsive genes, NK cells were isolated from spleen of WT and Runx3-/- mice . Ten samples (5 WT and 5 Runx3-/-) of freshly isolated NK cells were separately obtained from individual mice. Cells were cultured for 7 days with IL-2 or IL-15.

ORGANISM(S): Mus musculus

SUBMITTER: Joseph Lotem

PROVIDER: E-GEOD-50122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Runx3-mediated transcriptional program in cytotoxic lymphocytes.

Lotem Joseph J Levanon Ditsa D Negreanu Varda V Leshkowitz Dena D Friedlander Gilgi G Groner Yoram Y

PloS one 20131113 11

The transcription factor Runx3 is highly expressed in CD8(+) T and NK cytotoxic lymphocytes and is required for their effective activation and proliferation but molecular insights into the transcription program regulated by Runx3 in these cells are still missing. Using Runx3-ChIP-seq and transcriptome analysis of wild type vs. Runx3(-/-) primary cells we have now identified Runx3-regulated genes in the two cell types at both resting and IL-2-activated states. Runx3-bound genomic regions in both ...[more]

PMID: 24236182

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