Project description:Conditioned medium (CM) from bone marrow derived macrophages untreated or treated with LPS was collected and filtered through a 0.22-μm filter. The filtered CM was sequentially fractionated with 50-kDa and 100-kDa Amicon filters. The 50–100 kDa fraction of CM was analyzed by mass spectrometry.
2022-11-01 | PXD034550 | JPOST Repository
Project description:mouse bone marrow derived macrophages
Project description:Transcriptomic analysis of the temporal changes induced in mouse bone marrow derived macrophages (BMDMs) by the cytokine Interferon-beta over a timecourse of 0 to 24 hours of treatment. We set out to study the transcriptional events in mouse macrophages over time following stimulation with Interferon-beta. Mouse bone marrow derived macrophages were stimulated for 1, 2, 4, 8 and 24 hours with 10U/mL mouse interferon-beta or left untreated.
Project description:Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
Project description:Itaconate is a natural mild electrophile, able to modify free thiols in proteins upon activation. We submit two datasets investigating peptides modified by itaconate. Experiment001 contains data obtained from endogenous itaconate producing WT or itaconate incompetent Irg1-/- Bone marrow-derived macrophages stimulated with LPS for 24h. The second dataset from Experiment002 contains data obtained from WT Bone marrow-derived macrophages treated with media or 5 mM itaconic acid for 16h.
Project description:No.1 Vehicle control. Bone marrow derived macrophages were treated with vehicle control for 30 mins. Cells were then lysed and alkylated by IAM, followed by immunoprecipitation of STING protein and gel separation of the protein. After digestion and secondary alkylation by NEM, sample was submitted for MS analysis. No.2 Menadione treated sample. Bone marrow derived macrophages were treated with Menadione for 30 mins. Cells were then lysed and alkylated by IAM, followed by immunoprecipitation of STING protein and gel separation of the protein. After digestion and secondary alkylation by NEM, sample was submitted for MS analysis.