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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse pancreatic duct cell lines infected with Ngn3-expressing adenovirus

ABSTRACT: We have developed an in vitro model of Ngn3-dependent differentiation by infecting pancreatic duct cell lines with an Ngn3-expressing adenovirus. We used glass microarrays containing 18,000 mouse cDNAs and compared gene expression patterns in mPAC L20 cells infected with either AdCMV-gal (control) or AdCMV-ngn3. All comparisons were performed 48 h after viral infection, meaning that genes differentially expressed in Ngn3-expressing mPAC cells could include genes whose expression was directly or indirectly controlled by Ngn3.

ORGANISM(S): Mus musculus

SUBMITTER: Caroline Mrejen

PROVIDER: E-CBIL-15 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Proendocrine genes coordinate the pancreatic islet differentiation program in vitro.

Gasa Rosa R Mrejen Caroline C Leachman Nathaniel N Otten Marc M Barnes Michael M Wang Juehu J Chakrabarti Swarup S Mirmira Raghavendra R German Michael M

Proceedings of the National Academy of Sciences of the United States of America 20040830 36

In the developing pancreas, the basic helix-loop-helix (bHLH) protein Neurogenin3 (Ngn3) specifies which precursor cells ultimately will become endocrine cells and initiates the islet differentiation program. NeuroD1, a closely related bHLH protein and a downstream target of Ngn3, maintains the differentiation program initiated by Ngn3. We have developed an in vitro model of Ngn3-dependent differentiation by infecting pancreatic duct cell lines with an Ngn3-expressing adenovirus. We found that b ...[more]

PMID: 15340143

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INDUCTION OF PANCREATIC ISLET CELL DIFFERENTIATION BY THE NEUROGENIN-NEUROD CASCADE

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