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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation and transcription of mouse liver in the presence or absence of exogenous glucocorticoid to identify direct targets of the glucocorticoid receptor

ABSTRACT: The purpose of this study was to identify direct targets of the glucocorticoid receptor (GR) using an orthogonal analysis. An expression study of mouse livers in the presence or absence of exogenous glucocorticoid complemented a genome-wide location analysis on chromatin from the same livers. These were hybridized to the BCBC Mouse PancChip 5.0 and the Mouse PromoterChip BCBC-3.0 respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Klaus Kaestner

PROVIDER: E-CBIL-38 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Glucocorticoid receptor-dependent gene regulatory networks.

Phuc Le Phillip P Friedman Joshua R JR Schug Jonathan J Brestelli John E JE Parker J Brandon JB Bochkis Irina M IM Kaestner Klaus H KH

PLoS genetics 20050805 2

While the molecular mechanisms of glucocorticoid regulation of transcription have been studied in detail, the global networks regulated by the glucocorticoid receptor (GR) remain unknown. To address this question, we performed an orthogonal analysis to identify direct targets of the GR. First, we analyzed the expression profile of mouse livers in the presence or absence of exogenous glucocorticoid, resulting in over 1,300 differentially expressed genes. We then executed genome-wide location anal ...[more]

PMID: 16110340

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