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Effect of TCDD Exposure on purified splenic CD8 T cells

ABSTRACT: 8-12 week, female C57BL/6 mice were injected with 10 µg/kg TCDD or solvent control. CD8+ T cells from spleen were FACS purified and submitted to transcription profiling Keywords: TCDD, AHR, Immunosupression peripheral T cells, CD8 T cells, Toxicogenomics For each group 4 female C57BL/6 mice were treated, spleen surgically removed and single cell suspensions generated. Cell suspensions were pooled, and CD8 positive T cells purified (purity > 99%). RNA was isolated using TRIzol and amplified using the Ambion message AMP kit. Target lableing and hybridization was performed using Affymetrix standard protocols

ORGANISM(S): Mus musculus

SUBMITTER: Markus Frericks

PROVIDER: E-GEOD-10093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice.

Frericks Markus M Temchura Vladimir V VV Majora Marc M Stutte Susanne S Esser Charlotte C

Biological chemistry 20060901 9

The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-/- mice and wild-type mice; 390 genes, many of them immunotypic, were deregulated in AHR-deficient CD4 cells. TCDD-induced transcriptome changes correlated with the AHR expression l ...[more]

PMID: 16972790

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