Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from kidney and liver of an adult human male

ABSTRACT: mRNA expression differences between the liver and kidney of an adult male (homo sapien) were investigated using three technical replicates. The purpose of the experiment was to compare array data generated using Affymetrix with measures of expression obtained using RNAseq (a sequencing approach for measuring expression that utilizes Solexa technology). Keywords: kidney, liver RNA samples from the liver and kidney were extracted within 6 hours post mortem. Aliquots of mRNA were extracted and hybridized to three Affymetrix U133 Plus 2.0 arrays (three technical replicates). The purpose of the study was to compare the Affymetrix array data with expression results obtained from RNAseq (Solexa). This dataset is part of the TransQST collection.

ORGANISM(S): Homo sapiens

SUBMITTER: John Marioni 

PROVIDER: E-GEOD-11045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNA-seq: an assessment of technical reproducibility and comparison with gene expression arrays.

Marioni John C JC   Mason Christopher E CE   Mane Shrikant M SM   Stephens Matthew M   Gilad Yoav Y  

Genome research 20080611 9

Ultra-high-throughput sequencing is emerging as an attractive alternative to microarrays for genotyping, analysis of methylation patterns, and identification of transcription factor binding sites. Here, we describe an application of the Illumina sequencing (formerly Solexa sequencing) platform to study mRNA expression levels. Our goals were to estimate technical variance associated with Illumina sequencing in this context and to compare its ability to identify differentially expressed genes with  ...[more]

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