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EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection.

ABSTRACT: Little is known about the physiological role of the EBER1 and 2 nuclear RNAs during Epstein Barr viral infection. The cellular transcription response to EBER2 expression using the wild-type and an internal deletion mutant was determined. Significant changes in gene expression patterns were observed. A functional meta-analysis of the regulated genes points to inhibition of stress and immune responses, as well as activation of cellular growth and cytoskeletal reorganization as potential targets for EBER2 RNA. Different functions can be assigned to different parts of the RNA. These results provide new avenues to the understanding of EBER2 and EBV biology. Keywords: Overexpression of non-translated RNA Biological relicates, 3 conditions: empty expression vector (pUC-18), pUC-18-EBER2, pUC-18-EBER2-L2

ORGANISM(S): Homo sapiens

SUBMITTER: Arndt Benecke

PROVIDER: E-GEOD-11093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr Virus infection.

Eilebrecht Sebastian S Pellay François-Xavier FX Odenwälder Peter P Brysbaert Guillaume G Benecke Bernd-Joachim BJ Benecke Arndt A

BMC research notes 20081028

<h4>Background</h4>Little is known about the physiological role of the EBER1 and 2 nuclear RNAs during Epstein Barr viral infection. The EBERs are transcribed by cellular RNA Polymerase III and their strong expression results in 106 to 107 copies per EBV infected cell, making them reliable diagnostic markers for the presence of EBV. Although the functions of most of the proteins targeted by EBER RNAs have been studied, the role of EBERs themselves still remains elusive.<h4>Findings</h4>The cellu ...[more]

PMID: 18957101

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