Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The Applied Biosystems Tissue Gene Expression Database - Human Body Map. A whole genome survey of 32 Human Tissues

ABSTRACT: The Applied Biosystems Tissue Gene Expression Database, known as the Human Body Map, is a collection of gene expression profiles from 31 normal tissues and a Universal Human Reference RNA (Stratagene). The Human BodyMap was created using the Applied Biosystems Expression Array System. There were three replicates per tissue for a total of 96 microarrays. Keywords: Human tissue whole genome survey 31 human tissues from Clontech and 1 from Stratagene (UHR) were used to create the Applied Biosystems Human BodyMap (a.k.a.Tissue Gene Expression Database ). Digoxigenin-UTP-labeled cRNA was generated and linearly amplified from 1 µg of total RNA with three replicates per tissue, using the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit v 2.0 and kit protocol. Microarray hybridization, chemiluminescence detection, image acquisition, and analysis were performed on the on the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer using the Applied Biosystems Chemiluminescence Detection Kit and kit protocol. This dataset is part of the TransQST collection.

ORGANISM(S): Homo sapiens

SUBMITTER: Julie Blake

PROVIDER: E-GEOD-7905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A comprehensive functional analysis of tissue specificity of human gene expression.

Dezso Zoltán Z Nikolsky Yuri Y Sviridov Evgeny E Shi Weiwei W Serebriyskaya Tatiana T Dosymbekov Damir D Bugrim Andrej A Rakhmatulin Eugene E Brennan Richard J RJ Guryanov Alexey A Li Kelly K Blake Julie J Samaha Raymond R RR Nikolskaya Tatiana T

BMC biology 20081112

<h4>Background</h4>In recent years, the maturation of microarray technology has allowed the genome-wide analysis of gene expression patterns to identify tissue-specific and ubiquitously expressed ('housekeeping') genes. We have performed a functional and topological analysis of housekeeping and tissue-specific networks to identify universally necessary biological processes, and those unique to or characteristic of particular tissues.<h4>Results</h4>We measured whole genome expression in 31 human ...[more]

PMID: 19014478

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Dataset Information

The Applied Biosystems Tissue Gene Expression Database - Human Body Map. A whole genome survey of 32 Human Tissues

Publications

A comprehensive functional analysis of tissue specificity of human gene expression.

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