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Role of M. tuberculosis dosS and dosT in CO sensing

ABSTRACT: Transcriptional profiling of M. tuberculosis dosR and dosS mutants and wild type growing in log phase treated with carbon monoxide or nitric oxide versus untreated controls Keywords: Genetic modification Two condition experiment, DETANO treated cells at 100 uM or CO treated cells at 20000 ppm versus untreated controls for various M. tuberculosis mutants. Biological replicates: 2 replicates per dose, 1 array per replicate

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Michael Shiloh

PROVIDER: E-GEOD-11096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mycobacterium tuberculosis senses host-derived carbon monoxide during macrophage infection.

Shiloh Michael U MU Manzanillo Paolo P Cox Jeffery S JS

Cell host & microbe 20080501 5

Mycobacterium tuberculosis (MTB) expresses a set of genes known as the dormancy regulon in vivo. These genes are expressed in vitro in response to nitric oxide (NO) or hypoxia, conditions used to model MTB persistence in latent infection. Although NO, a macrophage product that inhibits respiration, and hypoxia are likely triggers in vivo, additional cues could activate the dormancy regulon during infection. Here, we show that MTB infection stimulates expression of heme oxygenase (HO-1) by macrop ...[more]

PMID: 18474359

Similar Datasets

Role of M. tuberculosis in CO sensing

Project description:This SuperSeries is composed of the following subset Series: GSE11095: Dose response of carbon monoxide treatment of M. tuberculosis GSE11096: Role of M. tuberculosis dosS and dosT in CO sensing Refer to individual Series

2008-05-28 | E-GEOD-10897 | biostudies-arrayexpress

Dose response of carbon monoxide treatment of M. tuberculosis

Project description:Transcriptional profiling of M. tuberculosis growing in log phase treated with various concentrations of carbon monoxide versus untreated controls Keywords: Dose response Two condition experiment, CO treated cells at 2000, 200 and 20 ppm versus untreated controls. Biological replicates: 2 replicates per dose, 1 array per replicate

2008-05-29 | E-GEOD-11095 | biostudies-arrayexpress

Role of M. tuberculosis dosS and dosT in CO sensing

Project description:Transcriptional profiling of M. tuberculosis dosR and dosS mutants and wild type growing in log phase treated with carbon monoxide or nitric oxide versus untreated controls Keywords: Genetic modification

2008-05-29 | GSE11096 | GEO

Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene

2008-08-08 | E-GEOD-12379 | biostudies-arrayexpress

Gene expression profile of M.tuberculosis espR (Rv3849) mutants containing N- or C-terminal mutations in the espR gene

Project description:Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of the following M.tb strains grown in log phase: Erdman strain of M.tb (WT), espR transposon mutant (R-), the espR transposon mutant complemented with the espR gene (R+), the espR mutant (R-) complemented with an espR gene containing an R8A amino acid subsitution (R- R8A), the espR mutant (R-) complemented with an espR gene containing an R21A amino acid subsitution (R- R21A), espR mutant (R-) complemented with an espR gene containing a C-terminal 10-amino acid truncation (R- delta10), and the espR mutant (R-) complemented with an espR gene containing a C-terminal 5-amino acid truncation (R- delta5).

2010-05-25 | E-GEOD-12380 | biostudies-arrayexpress

Gene expression profile of M.tuberculosis espR (Rv3849) mutant

2008-07-04 | E-GEOD-11696 | biostudies-arrayexpress

Secreted transcription factor controls Mycobacterium tuberculosis virulence

Project description:This SuperSeries is composed of the following subset Series: GSE11696: Gene expression profile of M.tuberculosis espR (Rv3849) mutant GSE12379: Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene GSE12380: Gene expression profile of M.tuberculosis espR (Rv3849) mutants containing N- or C-terminal mutations in the espR gene GSE12381: Comparative analysis of the gene expression profiles of M.tuberculosis espR (Rv3849) transposon and knockout mutants Refer to individual Series

2010-05-25 | E-GEOD-12382 | biostudies-arrayexpress

The unique roles of DosT and DosS in DosR regulon induction and Mycobacterium tuberculosis dormancy

Project description:In Mycobacterium tuberculosis, the sensor kinases DosT and DosS activate the transcriptional regulator DosR, resulting in the induction of the DosR regulon, important for anaerobic survival and perhaps latent infection. The individual and collective roles of these sensors has been postulated biochemically, but their roles have remained unclear in vivo. This work demonstrates distinct and additive roles for each sensor during anaerobic dormancy. Both sensors are necessary for wild type levels of DosR regulon induction, and concomitantly, full induction of the regulon is required for wild type anaerobic survival. In the anaerobic model, DosT plays an early role, responding to hypoxia. DosT then induces the regulon and with it DosS, which sustains and further induces the regulon. DosT then loses its functionality as oxygen becomes limited, and DosS alone maintains induction of the genes from that point forward. Thus, M. tuberculosis has evolved a system whereby it responds to hypoxic conditions in a stepwise fashion as it enters an anaerobic state.

2009-06-26 | GSE16811 | GEO

DosS Is Required for the Complete Virulence of Mycobacterium tuberculosis in Mice with Classical Granulomatous Lesions

Project description:Here we are including replicate mouse microarray data from Kramnik mice infected over the course of different time points with M. tuberculosis H37Rv and its isogenic mutants in dosR, dosS and dosT genes.

2014-12-11 | GSE64045 | GEO

The unique roles of DosT and DosS in DosR regulon induction and Mycobacterium tuberculosis dormancy

2009-06-26 | E-GEOD-16811 | biostudies-arrayexpress