Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

MiRNA Microarray Profiling of Nine Different Normal Human Tissues

ABSTRACT: We measured the expression of 470 human miRNAs in nine human tissues using Agilent DNA microarrays. miRNA microarrays were manufactured by Agilent Technologies (Santa Clara, CA), and contain 20-40 features targeting each of 470 human miRNAs (Agilent design ID 016436). Sequences of the 470 miRNAs were obtained from the Sanger miRBase, release 9.1. Labeling and hybridization of total RNA samples were performed according to the manufacturer’s protocol. 100ng total RNA was used as input into the labeling reaction, and the entire reaction was hybridized to the array for 20 hours at 55°C. The labeling and hybridizations of the nine human tissues were done 4-5 times using the same starting RNA sample for each tissue.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Ach

PROVIDER: E-GEOD-11806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Measuring microRNAs: comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods.

Ach Robert A RA Wang Hui H Curry Bo B

BMC biotechnology 20080911

<h4>Background</h4>Determining the expression levels of microRNAs (miRNAs) is of great interest to researchers in many areas of biology, given the significant roles these molecules play in cellular regulation. Two common methods for measuring miRNAs in a total RNA sample are microarrays and quantitative RT-PCR (qPCR). To understand the results of studies that use these two different techniques to measure miRNAs, it is important to understand how well the results of these two analysis methods cor ...[more]

PMID: 18783629

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Dataset Information

MiRNA Microarray Profiling of Nine Different Normal Human Tissues

Publications

Measuring microRNAs: comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods.

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