Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

MiRNA profiling of Melanoma Cell lines and Normal Melanocytes

ABSTRACT: To identify microRNAs potentially involved in melanomagenesis we compared microRNA transcription profiles between melanoma cell lines and cultured melanocytes. miRNA microarrays were manufactured by Agilent Technologies (Santa Clara, CA), and contain 20-40 features targeting each of 470 human miRNAs (Agilent design ID 016436). Sequences of the 470 miRNAs were obtained from the Sanger miRBase, release 9.1. Labeling and hybridization of total RNA samples were performed according to the manufacturer’s protocol. 100ng total RNA was used as input into the labeling reaction, and the entire reaction was hybridized to the array for 20 hours at 55°C. The labeling and hybridizations of the nine human tissues were done 4-5 times using the same starting RNA sample for each tissue. Total RNA was extracted from 51 melanoma cells and 2 pools of melanocytes using the QIAGEN miRNA kits.

ORGANISM(S): Homo sapiens

SUBMITTER: Mitchell Stark

PROVIDER: E-GEOD-25653 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor.

Boyle Glen M GM Woods Susan L SL Bonazzi Vanessa F VF Stark Mitchell S MS Hacker Elke E Aoude Lauren G LG Dutton-Regester Ken K Cook Anthony L AL Sturm Richard A RA Hayward Nicholas K NK

Pigment cell & melanoma research 20110407 3

To identify microRNAs potentially involved in melanomagenesis, we compared microRNA expression profiles between melanoma cell lines and cultured melanocytes. The most differentially expressed microRNA between the normal and tumor cell lines was miR-211. We focused on this pigment-cell-enriched miRNA as it is derived from the microphthalmia-associated transcription factor (MITF)-regulated gene, TRPM1 (melastatin). We find that miR-211 expression is greatly decreased in melanoma cells and melanobl ...[more]

PMID: 21435193

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Dataset Information

MiRNA profiling of Melanoma Cell lines and Normal Melanocytes

Publications

Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor.

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