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Transcription profiling of human HeLa cells by hsa-mir-26b overexpression

ABSTRACT: Total RNA samples from three biological replicates in which the hsa-mir-26b was overexpressed in HeLa cells were profiled by gene expression. As negative control, we used total RNA samples from HeLa cells transfected with cel-mir-67 Experiment Overall Design: hsa-miR-26b transfection

ORGANISM(S): Homo sapiens

SUBMITTER: Vincenzo Alessandro Gennarino

PROVIDER: E-GEOD-12091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA target prediction by expression analysis of host genes.

Gennarino Vincenzo Alessandro VA Sardiello Marco M Avellino Raffaella R Meola Nicola N Maselli Vincenza V Anand Santosh S Cutillo Luisa L Ballabio Andrea A Banfi Sandro S

Genome research 20081216 3

MicroRNAs (miRNAs) are small noncoding RNAs that control gene expression by inducing RNA cleavage or translational inhibition. Most human miRNAs are intragenic and are transcribed as part of their hosting transcription units. We hypothesized that the expression profiles of miRNA host genes and of their targets are inversely correlated and devised a novel procedure, HOCTAR (host gene oppositely correlated targets), which ranks predicted miRNA target genes based on their anti-correlated expression ...[more]

PMID: 19088304

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Profiling gene expression in HeLa cells by hsa-mir-26b overexpression

Project description:Total RNA samples from three biological replicates in which the hsa-mir-26b was overexpressed in HeLa cells were profiled by gene expression. As negative control, we used total RNA samples from HeLa cells transfected with cel-mir-67 Keywords: gene expression array-based (RNA / in situ oligonucleotide)

2008-12-17 | GSE12091 | GEO

Transcription profiling of human HeLa cells by hsa-mir-98 overexpression

Project description:Total RNA samples from three biological replicates in which the hsa-mir-98 was overexpressed in HeLa cells were profiled by gene expression. As negative control, we used total RNA samples from HeLa cells transfected with cel-mir-67 Experiment Overall Design: hsa-miR-98 transfection

2009-01-13 | E-GEOD-12092 | biostudies-arrayexpress

Profiling gene expression in HeLa cells by hsa-mir-98 overexpression

2008-12-17 | GSE12092 | GEO

Transcription profiling of human HeLa cells overexpressing hsa-mir-26b and hsa-mir-98 (super series)

Project description:This SuperSeries is composed of the following subset Series:; GSE12091: Profiling gene expression in HeLa cells by hsa-mir-26b overexpression; GSE12092: Profiling gene expression in HeLa cells by hsa-mir-98 overexpression Experiment Overall Design: Refer to individual Series

2009-05-13 | E-GEOD-12100 | biostudies-arrayexpress

Transcript expression levels of neonatal mouse cardiomyocytes mock-transfected, or transfected with cel-miR-67, hsa-miR-590-3p or hsa-miR-199a-3p

Project description:To identify the relevant targets of the selected miRNAs, we assessed global transcriptome changes by deep-sequencing total neonatal mouse cardiomyocyte RNA after transfection with hsa-miR-590-3p or hsa-miR-199a-3p Four condition experiment; one replicate per condition; mouse neonatal cardiomyocytes transfected with cel-miR-67, hsa-miR-590-3p and hsa-miR-199a-3p; samples collected 72 hours after transfection

2012-10-13 | E-GEOD-41538 | biostudies-arrayexpress

hsa-miR-503, hsa-miR-103, and hsa-miR-494 genome wide target profiles [RNA-Seq and RIP-Seq]

Project description:We constructed a genome wide target profile of hsa-miR-503, hsa-miR-103, and hsa-miR-494 by sequencing RNA isolated from Ago2 immunoprecipitations and total RNA samples following transfection of the respective miRNA in mature duplex form Examination of mRNA levels in HeLa cells and Ago2 immunoprecipitations from HeLa cells following miR-503, miR-103, or miR-494 mature duplex or control siRNA transfection

2015-02-04 | E-GEOD-64615 | biostudies-arrayexpress

RNA-seq profiles of rat cardiomyocytes overexpressing hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p or cel-miR-67

Project description:Myocardial regeneration is restricted to early postnatal life, when mammalian cardiomyocytes still retain the ability to proliferate. The molecular cues that induce cell cycle arrest of neonatal cardiomyocytes towards terminally differentiated adult heart muscle cells remain obscure. We report that the miR-106b~25cluster is higher expressed in the early postnatal myocardium and decreases in expression towards adulthood, especially under conditions of overload, and orchestrates the transition of cardiomyocyte hyperplasia towards cell cycle arrest and hypertrophy by virtue of its targetome. To identify the relevant targets of individual miRNAs in the miR-106b~15 cluster and elucidate the molecular mechanisms underlying the proliferative effects of this microRNA cluster, we assessed the global transcriptomic changes by deep-sequencing total neonatal mouse cardiomyocyte RNA after exogeneous transfection with hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p and compared the transcriptomic profiles to cardiomyocytes transfected with cel-miR-67, a control miRNA.

2021-06-25 | GSE178867 | GEO

Microarray analysis of the effect of hsa-miR-127-3p on the gene expression of Hela cells under the stimulation with IFN-α

Project description:In order to explore the effect of hsa-miR-127-3p on the gene expression downstream of type I interferon signaling pathway, we used IFN-α to treat Hela cells (1000U/ml for 8 h) which were previously transfected with hsa-miR-127-3p mimics or various controls (mock transfection, negative control mimics or hsa-miR-127-3p mutant mimics). RNAs from the Hela cells were subjected to microarray analysis.

2021-10-08 | E-MTAB-10804 | biostudies-arrayexpress

Hela hsa-miR-34a, b-5p, and c transcriptomes

Project description:Comparative transcriptome analysis of Hela cells transfected with hsa-miR-34a,b-5p or c.

2009-05-13 | GSE15957 | GEO

Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays

Project description:We screened for target genes of hsa-mir-199a-1 and hsa-mir-4423 by transcriptome profiling. The miRNAs were overexpressed in HeLa cells by transient transfection of precursor miRNA expression plasmids. High-density Clariom D arrays for human were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow.

2021-10-15 | GSE185859 | GEO

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