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Genome-wide mapping of RNA Pol III binding site (K562)

ABSTRACT: We report the results of chromatin immunoprecipitation following by high-thoughput tag sequencing (ChIP-Seq) using the GA II platform from Illumina for the human RNA Polymerase III in K562 cells. We have also generated a sequenced input DNA datasets for K562 cells. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Map binding sites of RNA Polymerase III in the genome of K562 cells. The K562 input data has been deposited in GEO as GSM325934.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Wilson 

PROVIDER: E-GEOD-19548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Close association of RNA polymerase II and many transcription factors with Pol III genes.

Raha Debasish D   Wang Zhong Z   Moqtaderi Zarmik Z   Wu Linfeng L   Zhong Guoneng G   Gerstein Mark M   Struhl Kevin K   Snyder Michael M  

Proceedings of the National Academy of Sciences of the United States of America 20100205 8

Transcription of the eukaryotic genomes is carried out by three distinct RNA polymerases I, II, and III, whereby each polymerase is thought to independently transcribe a distinct set of genes. To investigate a possible relationship of RNA polymerases II and III, we mapped their in vivo binding sites throughout the human genome by using ChIP-Seq in two different cell lines, GM12878 and K562 cells. Pol III was found to bind near many known genes as well as several previously unidentified target ge  ...[more]

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