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Effects of extensive fasting on hepatic transcription

ABSTRACT: Temporally restricted feeding has a profound effect on the hepatic circadian clock. While the circadian clock is largely unaffected by by extensive fasting, many transcripts are known to be affected by a fasting paradigm. This dataset shows the effect of extensive fasting on dynamic gene expression in the liver C57/B6 mice were entrained to ad libitum feeding schedule for two weeks. They were then released into constant were food was withdrawn at CT16. On the second day in constant darkness tissue was collected at the indicated timepoints. Total RNA was extracted and 5ug of total RNA was used for the standard Affymetrix protocol of amplification, labeling and hybridization

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Vollmers

PROVIDER: E-GEOD-13064 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Time of feeding and the intrinsic circadian clock drive rhythms in hepatic gene expression.

Vollmers Christopher C Gill Shubhroz S DiTacchio Luciano L Pulivarthy Sandhya R SR Le Hiep D HD Panda Satchidananda S

Proceedings of the National Academy of Sciences of the United States of America 20091125 50

In mammals, the circadian oscillator generates approximately 24-h rhythms in feeding behavior, even under constant environmental conditions. Livers of mice held under constant darkness exhibit circadian rhythm in abundance in up to 15% of expressed transcripts. Therefore, oscillations in hepatic transcripts could be driven by rhythmic food intake or sustained by the hepatic circadian oscillator, or a combination of both. To address this question, we used distinct feeding and fasting paradigms on ...[more]

PMID: 19940241

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