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Transcription profiling of human wild type or domain swap mutants of A-Myb and c-Myb transcription factors in MCF-7 cells or pimary lung epithelial cells or fibroblasts Myb specificity determinants

ABSTRACT: Recombinant adenovirus vectors were used to express wild type or domain swap mutants of A-Myb and c-Myb transcription factors in MCF-7 cells or pimary lung epithelial cells or fibroblasts. The results show that Myb proteins have extreme context specificity and identify sub-domains responsible for the activation of specific sets of target genes.

ORGANISM(S): Homo sapiens

SUBMITTER: Scott Ness

PROVIDER: E-GEOD-1318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Positive and negative determinants of target gene specificity in myb transcription factors.

Lei Wanli W Rushton John J JJ Davis Lisa M LM Liu Fan F Ness Scott A SA

The Journal of biological chemistry 20040421 28

The A-Myb and c-Myb transcription factors share a highly conserved DNA-binding domain and activate the same promoters in reporter gene assays. However, the two proteins have distinct biological activities, and expressing them individually in human cells leads to the activation of distinct sets of endogenous genes, suggesting that each protein has a unique transcriptional specificity. Here, the structural and functional features of the Myb proteins were compared, using assays of endogenous gene e ...[more]

PMID: 15105423

Similar Datasets

Myb specificity determinants

Project description:Recombinant adenovirus vectors were used to express wild type or domain swap mutants of A-Myb and c-Myb transcription factors in MCF-7 cells or pimary lung epithelial cells or fibroblasts. The results show that Myb proteins have extreme context specificity and identify sub-domains responsible for the activation of specific sets of target genes. Keywords = Myb proteins Keywords = oncogenes Keywords = transcription Keywords = gene activation Keywords: ordered

2004-04-16 | GSE1318 | GEO

Transcription profiling of human monocytes infected with adenovirus expressing either c-Myb or v-Myb

Project description:The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in primary human monocytes using recombinant adenovirus vectors. All the samples were compared to cells infected with a control adenovirus expressing only GFP. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. Experiment Overall Design: 2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

2007-12-12 | E-GEOD-2816 | biostudies-arrayexpress

Transcription profiling of human MCF7 cells infected with adenovirus expressing c-Myb, v-Myb or the hybrid construct, 3MutC

Project description:The transcriptional activities of c-Myb and its oncogenic variant v-Myb were compared by expressing them in human MCF7 cells using recombinant adenovirus vectors. A hybrid construct, 3Mutc, which is a variant of c-Myb harboring three v-Myb-derived DNA binding domain mutations was also analyzed. All the samples were compared to cells infected with a control adenovirus. The results showed that v-Myb, which differs from c-Myb only by N- and C-terminal deletions and eleven amino acid substitutions, has a qualitatively different transcriptional activity. Experiment Overall Design: 2 replicates of each type were analyzed. Replicates were performed independently, more than a week apart.

2007-12-12 | E-GEOD-2815 | biostudies-arrayexpress

Expression data of cultured MCF10A cells transduced with MYB or MYB-NFIB expression vectors

Project description:This experiment investigates differences between control cells (empty vector) and cells with MYB or MYB-NFIB (M14N9) overexpression using MSCV vectors

2020-01-31 | GSE136095 | GEO

cMyb and vMyb in human monocytes

2005-06-14 | GSE2816 | GEO

cMyb and vMyb in MCF7 cells

2005-06-14 | GSE2815 | GEO

Sub-populations of c-Myb in MCF-7 cells

Project description:We compared two independent c-Myb antibodies during cellular confluence and estrogen stimulation to determine endogenous c-Myb target genes. Interestingly, we discovered that c-Myb specificity was estrogen dependent and multiple antibodies recognize distinct sets of endogenous targets suggesting that epitope masking and domains within the c-Myb protein play a critical role in the activity of the c-Myb protein.

2010-05-01 | GSE18706 | GEO

MYB-regulated pancreatic cancer secretome

Project description:Earlier we have shown important roles of MYB in pancreatic tumor pathobiology. To better understand the role of MYB in the tumor microenvironment and identify MYB-associated secreted biomarker proteins, we conducted mass spectrometry analysis of the secretome from MYB-modulated and control pancreatic cancer cell lines. We also performed in silico analyses to determine MYB-associated biofunctions, gene networks and altered biological pathways. We further investigated the secreted proteins for their potential biomarker properties.

2021-09-08 | PXD015126 | Pride

Expression data of cultured MCF10A cells transduced with MYB or MYB-NFIB expression vectors

Project description:Expression data of cultured MCF10A cells transduced with MYB or MYB-NFIB expression vectors

| PRJNA561286 | ENA

MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter

Project description:Most E2F-binding sites repress transcription through the recruitment of Retinoblasoma (RB) family members until the end of the G1 cell-cycle phase. Although the MYB promoter contains an E2F-binding site, its transcription is activated shortly after the exit from quiescence, before RB family members inactivation, by unknown mechanisms. We had previously uncovered a nuclear factor distinct from E2F, Myb-sp, whose DNA-binding site overlapped the E2F element and had hypothesized that this factor might overcome the transcriptional repression of MYB by E2F-RB family members. We have purified Myb-sp and discovered that Myc-associated zinc finger proteins (MAZ) are major components. We show that various MAZ isoforms are present in Myb-sp and activate transcription via the MYB-E2F element. Moreover, while forced RB or p130 expression repressed the activity of a luciferase reporter driven by the MYB-E2F element, co-expression of MAZ proteins not only reverted repression, but also activated transcription. Finally, we show that MAZ binds the MYB promoter in vivo, that its binding site is critical for MYB transactivation, and that MAZ knockdown inhibits MYB expression during the exit from quiescence. Together, these data indicate that MAZ is essential to bypass MYB promoter repression by RB family members and to induce MYB expression.

2017-10-04 | PXD006857 | Pride

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