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Analysis of Spt16 depletion

ABSTRACT: Transcription rate analysis in Yeast by means of GRO, mRNA amount and ChIP-on-chip during the depletion of Spt16p. Keywords: Genomic run-on GRO ChIP-chip Transcription rate analysis by means of GRO and mRNA amount (RA) of three independent replicates during the depletion of Spt16p (Control and 5 & 7 hours after the depletion). Each time point replicate has been hybridized on a different macroarray (F14-F16). ChIP-on-chip analysis of Spt16 were done during exponential grow in YPD.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jose Garcia-Martinez

PROVIDER: E-GEOD-14082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulon-specific control of transcription elongation across the yeast genome.

Pelechano Vicent V Jimeno-González Silvia S Rodríguez-Gil Alfonso A García-Martínez José J Pérez-Ortín José E JE Chávez Sebastián S

PLoS genetics 20090821 8

Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classe ...[more]

PMID: 19696888

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