Unknown,Transcriptomics,Genomics,Proteomics

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Diabetic lung


ABSTRACT: Effect of type 1 diabetes (induced by streptozotocin 60 mg/kg) on lung gene expression. Wistar rats, male. At age 8 weeks control rats got IP buffer, diabetic rats got streptozotocin. At age 12 weeks animals were anesthetized and lungs removed. RNA was extracted with Trizol, and gene expression array analysis was performed using Affymetrix RAE 230A microarrays according to the directions from the manufacturer. Arrays were scanned using a Hewlett Packard Gene Array scanner, and analyzed with Affymetrix MAS 5.0 software. Expression levels reported are the output from the MAS software. Control rats (n=5) vs diabetic rats (n=7) Each sample is from a different animal (thus these are biological replicates). Samples were NOT pooled. Thus each sample corresponds to one animal.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Erik van Lunteren 

PROVIDER: E-GEOD-15900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Alterations in lung gene expression in streptozotocin-induced diabetic rats.

van Lunteren Erik E   Moyer Michelle M   Spiegler Sarah S  

BMC endocrine disorders 20140115


<h4>Background</h4>Diabetes profoundly affects gene expression in organs such as heart, skeletal muscle, kidney and liver, with areas of perturbation including carbohydrate and lipid metabolism, oxidative stress, and protein ubiquitination. Type 1 diabetes impairs lung function, but whether gene expression alterations in the lung parallel those of other tissue types is largely unexplored.<h4>Methods</h4>Lung from a rat model of diabetes mellitus induced by streptozotocin was subjected to gene ex  ...[more]

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