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Effect of phenylalanine-rich peptide on growth and survival of Mycobacterium tuberculosis H37Rv (Mtb)

ABSTRACT: During our efforts to isolate potantial binding partners of Esat6, we isolated few peptides rich in phenylalanine residues that strongly interacted with Esat6. All peptides were less than fifty amino acids in length, One of them, Hcl1, when expressed in mycobacteria showed significant retardation in growth and survival within macrophages. Microarray analysis showed that Hcl1 affects a host of genes and cellular pathways. RNA was isolated from exponentially growing mycobacteria containing either plasmid vector pVV16 encoding peptide or vector pVV16 alone. Comparisons were made between Experimental (Mtb/Hcl1) and control (Mtb/pVV16) samples by extracting raw intensity values from multiple arrays.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: krishan kumar 

PROVIDER: E-GEOD-16618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Phenylalanine-rich peptides potently bind ESAT6, a virulence determinant of Mycobacterium tuberculosis, and concurrently affect the pathogen's growth.

Kumar Krishan K   Tharad Megha M   Ganapathy Swetha S   Ram Geeta G   Narayan Azeet A   Khan Jameel Ahmad JA   Pratap Rana R   Ghosh Anamika A   Samuchiwal Sachin Kumar SK   Kumar Sushil S   Bhalla Kuhulika K   Gupta Deepti D   Natarajan Krishnamurthy K   Singh Yogendra Y   Ranganathan Anand A  

PloS one 20091110 11

<h4>Background</h4>The secretory proteins of Mycobacterium tuberculosis (M. tuberculosis) have been known to be involved in the virulence, pathogenesis as well as proliferation of the pathogen. Among this set, many proteins have been hypothesized to play a critical role at the genesis of the onset of infection, the primary site of which is invariably the human lung.<h4>Methodology/principal findings</h4>During our efforts to isolate potential binding partners of key secretory proteins of M. tube  ...[more]

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