Identification of genes that are regulated by the A and B isoforms of the human progesterone receptor
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: NIEHS Microarray Core
PROVIDER: E-GEOD-18276 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Multimodal regulation of E2F1 gene expression by progestins.
Wade Hilary E HE Kobayashi Sakiko S Eaton Matthew L ML Jansen Michelle S MS Lobenhofer Edward K EK Lupien Mathieu M Geistlinger Timothy R TR Zhu Wencheng W Nevins Joseph R JR Brown Myles M Otteson Deborah C DC McDonnell Donald P DP
Molecular and cellular biology 20100201 8
An analysis of mRNA expression in T47D breast cancer cells treated with the synthetic progestin R5020 revealed a subset of progesterone receptor (PR) target genes that are enriched for E2F binding sites. Following up on this observation, we determined that PR-B acts in both direct and indirect manners to positively upregulate E2F1 expression in T47D cells. The direct effects of PR on E2F1 expression were confirmed by chromatin immunoprecipitation (ChIP) analysis, which indicated that the agonist ...[more]
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