Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from PonA stimulated transfected U373 Cells


ABSTRACT: Induction of proximal components of the PI3-K/AKT pathway stimulates the expression of several genes, including several genes in the JAK/STAT pathway, documenting a crosstalk between these two important pathways. These changes were accompnied by nuclear translocation of certain transcription factors as well. we measured levels of RNA transcripts using the Human Genome U95 Av2 geneChip microarrays. We used an ecdysone-inducible mammalian expression system.

ORGANISM(S): Homo sapiens

SUBMITTER: F Uckun 

PROVIDER: E-GEOD-18798 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

STAT3 is a substrate of SYK tyrosine kinase in B-lineage leukemia/lymphoma cells exposed to oxidative stress.

Uckun Fatih M FM   Qazi Sanjive S   Ma Hong H   Tuel-Ahlgren Lisa L   Ozer Zahide Z  

Proceedings of the National Academy of Sciences of the United States of America 20100129 7


We provide unprecedented genetic and biochemical evidence that the antiapoptotic transcription factor STAT3 serves as a substrate for SYK tyrosine kinase both in vitro and in vivo. Induction of SYK in an ecdysone-inducible mammalian expression system results in STAT3 activation, as documented by tyrosine phosphorylation and nuclear translocation of STAT3, as well as amplified expression of several STAT3 target genes. STAT3 activation after oxidative stress (OS) is strongly diminished in DT40 chi  ...[more]

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