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Ovary small RNA

ABSTRACT: We sequenced small RNA from total RNA samples in two siRNA-pathway mutants in fly ovaries. This submission comes from a modENCODE project. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster Canton S ovaries. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-25570 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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R2D2 organizes small regulatory RNA pathways in Drosophila.

Okamura Katsutomo K Robine Nicolas N Liu Ying Y Liu Qinghua Q Lai Eric C EC

Molecular and cellular biology 20101206 4

Drosophila microRNAs (miRNAs) and small interfering RNAs (siRNAs) are generally produced by different Dicer enzymes (Dcr-1 and Dcr-2) and sorted to functionally distinct Argonaute effectors (AGO1 and AGO2). However, there is cross talk between these pathways, as highlighted by the recognition that Drosophila miRNA* strands (the partner strands of mature miRNAs) are generated by Dcr-1 but are preferentially sorted to AGO2. Here, we show that a component of the siRNA loading complex, R2D2, is esse ...[more]

PMID: 21135122

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