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Drosophila melanogaster small RNA sequence: female heads

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster female head. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt Quality scores in the supplementary file for GSM240749 are undefined. Quality of the bases assessed by (1) identifying for the sequenced linker, which is a known sequence, and (2) mapping the clipped sequence to the genome and taking only perfect hits.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Wei-Jen Chung

PROVIDER: E-GEOD-10790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functionally distinct regulatory RNAs generated by bidirectional transcription and processing of microRNA loci.

Tyler David M DM Okamura Katsutomo K Chung Wei-Jen WJ Hagen Joshua W JW Berezikov Eugene E Hannon Gregory J GJ Lai Eric C EC

Genes & development 20080101 1

Many microRNA (miRNA) loci exhibit compelling hairpin structures on both sense and antisense strands; however, the possibility that a miRNA gene might produce functional species from its antisense strand has not been examined. We report here that antisense transcription of the Hox miRNA locus mir-iab-4 generates the novel pre-miRNA hairpin mir-iab-8, which is then processed into endogenous mature miRNAs. Sense and antisense iab-4/iab-8 miRNAs are functionally distinguished by their distinct doma ...[more]

PMID: 18172163

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