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Small RNA from S2 cells (S2-NP)

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2 cells obtained from Norbert Perrimon's lab. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-14849 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Distinct mechanisms for microRNA strand selection by Drosophila Argonautes.

Okamura Katsutomo K Liu Na N Lai Eric C EC

Molecular cell 20091101 3

In Drosophila, miRNA strands are predominantly sorted into AGO1 to regulate seed-matched target transcripts, while their partner miRNA* strands are thought to be mostly degraded. Here, we report that Drosophila Argonautes exhibit different strand preferences for miRNA duplexes, and that in particular, many miRNA* species accumulate in the RNAi effector AGO2. AGO2-loaded miRNA* species require canonical RNAi factors for their accumulation, are efficiently 3' modified, and are preferentially activ ...[more]

PMID: 19917251

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