Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

36 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of sfh deletion on Salmonella Typhimurium gene expression

ABSTRACT: Transcriptional profiling of Salmonella Typhimurium strains SL1344 (pSfR27) and SL1344 (pSfR27) delta sfh to identify 'Sfh-dependent' transcripts Two condition experiment in which the transcriptomes of wild-type SL1344 (pSfR27) and SL1344 (pSfR27) delta sfh were compared to identify 'Sfh-dependent' transcripts

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Shane Dillon

PROVIDER: E-GEOD-19230 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression analysis of Salmonella enterica serovar Typhimurium strain SL1344 swap

Project description:We performed transcriptome abundance analysis of Salmonella Typhimurium strain SL1344 swap which has been genetically engineered to express the hns open-reading frame from the stpA promoter and the stpA open reading frame from the hns promoter. This strain is designated SL1344(swap). Transcript abundance was compared with that of wild-type SL1344. This comparison was performed to determine the effect of chromosome location of the expression of two related global regulators and how alterations to their expression patterns would impact on their regulons. Three independent RNA samples were harvested from wild-type SL1344 and SL1344(swap) grown to exponential phase (OD600nm = 0.3) and hybridized to a microarray containing multiple probes for each of the SL1344 open reading frames. Please note that the original raw data file for the 'SL1344_wildtype_BR2' sample is unavailable, however, the 'wildtype SL1344 BR2.gpr' contains the raw data missing only the header information.

2013-11-09 | E-GEOD-52235 | biostudies-arrayexpress

Identification of Sfh and H-NS binding sites in the Salmonella Typhimurium genome

Project description:The global regulator H-NS represses transcription in gram negative bacteria. Sfh is a homologue of H-NS and is encoded by plasmid pSfR27. Sfh provides a 'stealth' function that allows pSfR27 to be transmitted to a new host without disrupting the competitive fitness of the new host We used ChIP-on-chip to profile Sfh (3xFLAG-tagged) and H-NS binding sites in Salmonella Typhimurium strain SL1344 and found that Sfh provides its 'stealth' function by targeting a sub-set of H-NS bound genes that display reduced levels of H-NS occupancy with the SL1344 chromosome upon acquisition of plasmid pSfR27 Identification of Sfh binding sites in strains SL1344 (pSfR27) and SL1344 hns (pSfR27) as well as identification of H-NS binding sites in strains SL1344, SL1344 (pSfR27), and SL1344 (pSfR27) sfh

2010-05-18 | E-GEOD-19231 | biostudies-arrayexpress

Sfh and H-NS binding sites in the Salmonella Typhimurium genome

Project description:This SuperSeries is composed of the following subset Series: GSE19230: The effect of sfh deletion on Salmonella Typhimurium gene expression GSE19231: Identification of Sfh and H-NS binding sites in the Salmonella Typhimurium genome Refer to individual Series

2010-05-18 | E-GEOD-19356 | biostudies-arrayexpress

ChIP-chip analysis of CspC binding in Salmonella enterica serovar Typhimurium strain SL1344

Project description:We performed Chromatin Immunoprecipitation (ChIP) and microarray hybridization analysis of CspC binding in Salmonella Typhimurium strain SL1344 which has been genetically engineered to express a 3xFLAG tagged CspC protein. Chromatin samples were prepared from SL1344 CspC 3xFLAG grown to exponential phase (OD600nm = 0.2). CspC FLAG ChIP and mock normal mouse IgG ChIP reactions were carried out. The purified ChIP DNA samples were hybridized to SL1344 tiling microarrays.

2015-02-26 | E-GEOD-66288 | biostudies-arrayexpress

ChIP-chip of RNA Polymerase (RNAP) and RpoD in Salmonella enterica serovar Typhimurium strain SL1344

Project description:ChIP-on-chip analysis of RNAP and RpoD binding to the Salmonella enterica serovar Typhimurium chromosome demonstrated a high degree of overlap between RNAP and RpoD binding and provided us with important insights into the global distribution of these factors. Furthermore this data was correlated with information on the location of 1873 transcription start sites identified by RNA-Seq technology, thereby providing a detailed transcriptional map of Salmonella Typhimurium. Analysis of RNAP, RNAP-Rifampicin and and RpoD binding in Luria Broth (LB)

2012-02-16 | E-GEOD-35827 | biostudies-arrayexpress

ChIP-chip of LeuO, H-NS and RNA Polymerase (RNAP) in Salmonella enterica serovar Typhimurium strain SL1344

Project description:The LysR family transcription factor LeuO is believed to antagonize the global repressor H-NS. ChIP-on-chip analysis of LeuO, H-NS and RNAP binding to the Salmonella enterica serovar Typhimurium chromosome demonstrated a high degree of overlap between LeuO and H-NS regulated genes. Furthermore LeuO binding was associated with RNA polymerase recruitment, indicating a role for LeuO in activating transcription. Analysis of LeuO, H-NS and RNA Polymerase binding in Low-phosphate media (LPM)

2012-02-16 | E-GEOD-35826 | biostudies-arrayexpress

Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a ramA over-expressing strain

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic ramA gene knockout mutant which contained ramA upon an IPTG-inducible over-expression plasmid.

2009-12-31 | E-MEXP-2195 | biostudies-arrayexpress

Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 ramA gene knockout

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and SL1344 lacking a functional ramA were compared

2009-12-31 | E-MEXP-2193 | biostudies-arrayexpress

Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 soxS knockout

Project description:The transcriptome of Salmonella enterica serovar Typhimurium SL1344 was compared to that of SL1344 with soxS knocked out.

2010-12-01 | E-MEXP-2888 | biostudies-arrayexpress

Comparison of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 acrF gene knockout

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic acrF::aph mutant were compared.

2011-06-01 | E-MEXP-2976 | biostudies-arrayexpress