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Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a ramR gene knockout

ABSTRACT: The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic ramR::aph mutant were compared.

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Andrew Bailey

PROVIDER: E-MEXP-2194 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RamA, a member of the AraC/XylS family, influences both virulence and efflux in Salmonella enterica serovar Typhimurium.

Bailey Andrew M AM Ivens Al A Kingsley Rob R Cottell Jennifer L JL Wain John J Piddock Laura J V LJ

Journal of bacteriology 20100115 6

The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 lacking a functional ramA or ramR or with plasmid-mediated high-level overexpression of ramA were compared to those of the wild-type parental strain. Inactivation of ramA led to increased expression of 14 SPI-1 genes and decreased expression of three SPI-2 genes, and it altered expression of ribosomal biosynthetic genes and several amino acid biosynthetic pathways. Furthermore, disruption of ramA led to decreased survival withi ...[more]

PMID: 20081028

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Transcription profiling of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 ramA gene knockout

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and SL1344 lacking a functional ramA were compared

2009-12-31 | E-MEXP-2193 | biostudies-arrayexpress

Comparison of Salmonella enterica serovar Typhimurium SL1344 and an acrD gene knockout

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic acrD::aph mutant were compared.<br><br>

2011-06-01 | E-MEXP-2975 | biostudies-arrayexpress

Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 marA knockout

Project description:The transcriptome of Salmonella enterica serovar Typhimurium SL1344 was compared to an isogenic mutant in which marA was deleted.

2010-12-01 | E-MEXP-2886 | biostudies-arrayexpress

Comparison of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 acrF gene knockout

Project description:The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 and an isogenic acrF::aph mutant were compared.

2011-06-01 | E-MEXP-2976 | biostudies-arrayexpress

Transcription profiling by array of Salmonella enterica serovar Typhimurium SL1344 and a SL1344 soxS knockout

Project description:The transcriptome of Salmonella enterica serovar Typhimurium SL1344 was compared to that of SL1344 with soxS knocked out.

2010-12-01 | E-MEXP-2888 | biostudies-arrayexpress

Transcription profiling of Salmonella enterica serovar Typhimurium to investigate the disruption of tolC on the transcriptome

Project description:Analysis of the effect of disruption of tolC on the transcriptome of Salmonella enterica serovar Typhimurium

2009-06-03 | E-MEXP-1982 | biostudies-arrayexpress

Transcription profiling of Salmonella enterica serovar Typhimurium to investigate the effects of acrB duisruption

Project description:Analysis of effect of disruption of acrB on the transcriptome of Salmonella enterica serovar Typhimurium

2009-06-03 | E-MEXP-1981 | biostudies-arrayexpress

Transcription profiling of wild type and acrA inactivated Salmonella enterica to investigate the role of acrA in antibiotic resistance and pathogenicity

Project description:To investigate the role of acrA in antibiotic resistance and pathogenicity this gene was inactivated by insertion of a kanamycin cassette in strain SL1344 and the results on the transcriptome determined.

2008-12-31 | E-MEXP-1832 | biostudies-arrayexpress

Transcription profiling of Salmonella enterica serovar Typhimurium exposure to triclosan

Project description:S. Typhimurium SL1344 was exposed to the minimum inhibitory concentration of triclosan and the transcriptomic response analysed.

2009-08-28 | E-MEXP-2078 | biostudies-arrayexpress

Minimal vs LB media comparison

Project description:The type of bacterial culture medium is an important consideration during design of any experimental protocol. The aim of this study was to understand the impact of medium choice on bacterial gene expression and physiology by comparing the transcriptome of Salmonella enterica SL1344 after growth in the widely used LB broth or the rationally designed MOPS minimal medium. Transcriptomics showed that after growth in MOPS minimal media, compared to LB, there was increased expression of 42 genes involved in amino acid synthesis and 23 genes coding for ABC transporters. Seven flagellar genes had decreased expression after growth in MOPS minimal medium and this correlated with a decreased motility. In both MOPS minimal medium and MEM expression of genes from SPI-2 was increased and the adhesion of S. Typhimurium to intestinal epithelial cells was higher compared to the levels after growth in LB. However, SL1344 invasion was not significantly altered by growth in either MOPs minimal media or MEM. Expression of SPI-2 was also measured using chromosomal GFP reporter fusions followed by flow cytometry which showed, for the first time, that the reduction in SPI-2 transcript after growth in different media related to a reduction in the proportion of the bacterial population expressing SPI-2. These data highlight the profound differences in the global transcriptome after in vitro growth in different media and show that choice of medium should be considered carefully during experimental design, particularly when virulence related phenotypes are being measured.

2014-07-31 | E-MTAB-1135 | biostudies-arrayexpress

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