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ChIP-Seq sequencing of novel interactors of H3K4me3, H3K36me3 and H3K9me3

ABSTRACT: This study describes the epigenetic profiling of the novel interactors of H3K4me3, H3K36me3 or H3K9me3. The interactors were ChIP-Seq profiled by their GFP tag in stably transfected HeLa (Kyoto) cells. The interactors include GATAD1, Sgf29, BAP18, TRRAP, PHF8, N-PAC and LRWD1 (including replicates), as well as an GFP ChIP-Seq profile on non-transfected HeLa cells (negative control). Also included are the profiles of the histone modifications themselves (H3K4me3, H3K27me3, H3K9me3, H3K36me3, H3K9/14Ac and H3K79me3) ChIP-Seq profiling of 8 proteins by their GFP tag in stably transfected cells HeLa (Kyoto) cells, 6 replicas, as well as ChIP-Seq profiling of 6 histone modifications in wt HeLa (Kyoto) cells

ORGANISM(S): Homo sapiens

SUBMITTER: Hendrik Marks 

PROVIDER: E-GEOD-20303 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.

Vermeulen Michiel M   Eberl H Christian HC   Matarese Filomena F   Marks Hendrik H   Denissov Sergei S   Butter Falk F   Lee Kenneth K KK   Olsen Jesper V JV   Hyman Anthony A AA   Stunnenberg Henk G HG   Mann Matthias M  

Cell 20100901 6

Trimethyl-lysine (me3) modifications on histones are the most stable epigenetic marks and they control chromatin-mediated regulation of gene expression. Here, we determine proteins that bind these marks by high-accuracy, quantitative mass spectrometry. These chromatin "readers" are assigned to complexes by interaction proteomics of full-length BAC-GFP-tagged proteins. ChIP-Seq profiling identifies their genomic binding sites, revealing functional properties. Among the main findings, the human SA  ...[more]

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