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Global gene expression profile of Haemophilus ducreyi 35000HPcpxRpML125 relative to 35000HPcpxRpLS88

ABSTRACT: Haemophilus ducreyi 35000HPcpxRpML125 which over-expresses CpxR in a CpxR deletion background, was compared to it vector alone counterpart (35000HPcpxRpLS88) in hopes to elucidate members of the CpxRA regulon. Haemophilus ducreyi 35000HPcpxRpML125 and 35000HPcpxRpLS88 were grown in Columbia broth. After 8 hr of growth, total RNA was isolated and processed for DNA microarray analysis. This study includes 3 biological replicates (paired samples), two of which were selected for dye swap.

ORGANISM(S): Haemophilus ducreyi

SUBMITTER: Eric Hansen

PROVIDER: E-GEOD-21789 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of the CpxRA regulon in Haemophilus ducreyi.

Labandeira-Rey Maria M Brautigam Chad A CA Hansen Eric J EJ

Infection and immunity 20100830 11

The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Rey, J. R. Mock, and E. J. Hansen, Infect. Immun. 77:3402-3411, 2009). In the present study, the H. ducreyi CpxR and CpxA proteins were shown to closely resemble those of other well-s ...[more]

PMID: 20805330

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Haemophilus ducreyi 35000HPcpxA gene expression relative to 35000HP

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Mutant2

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