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Global gene expression profile of Haemophilus ducreyi 35000HPcpxRpML125 relative to 35000HPcpxRpLS88

ABSTRACT: Haemophilus ducreyi 35000HPcpxRpML125 which over-expresses CpxR in a CpxR deletion background, was compared to it vector alone counterpart (35000HPcpxRpLS88) in hopes to elucidate members of the CpxRA regulon.

ORGANISM(S): [Haemophilus] ducreyi [Haemophilus] ducreyi 35000HP

PROVIDER: GSE21789 | GEO | 2011/05/03

SECONDARY ACCESSION(S): PRJNA127319

REPOSITORIES: GEO

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Global gene expression profile of Haemophilus ducreyi 35000HPcpxRpML125 relative to 35000HPcpxRpLS88

Project description:Haemophilus ducreyi 35000HPcpxRpML125 which over-expresses CpxR in a CpxR deletion background, was compared to it vector alone counterpart (35000HPcpxRpLS88) in hopes to elucidate members of the CpxRA regulon. Haemophilus ducreyi 35000HPcpxRpML125 and 35000HPcpxRpLS88 were grown in Columbia broth. After 8 hr of growth, total RNA was isolated and processed for DNA microarray analysis. This study includes 3 biological replicates (paired samples), two of which were selected for dye swap.

2011-05-03 | E-GEOD-21789 | biostudies-arrayexpress

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RpoE-dependent transcriptomes of Haemophilus ducreyi

Project description:To potentially understand how H. ducreyi responds to membrane stress, here we defined RpoE-dependent genes using RNA-Seq. We identified 180 RpoE-dependent genes, of which 98% were upregulated; a major set of these genes encodes homologues of envelope maintenance and repair factors. We also identified and validated a putative RpoE promoter consensus sequence, which was enriched in the majority of RpoE-dependent targets. Comparison of RpoE-dependent genes to those controlled by CpxR showed that each transcription factor regulated a distinct set of genes. Given that RpoE activated a large number of genes encoding envelope maintenance and repair factors and CpxRA represses genes encoding envelope-localized proteins, these data suggest that RpoE and CpxRA appear to play distinct yet complementary roles in regulating envelope homeostasis in H. ducreyi. RNA of Haemophilus ducreyi 35000HP wild-type strain containing a rpoE inducible plasmid and wild-type strain containing a control plasmid were collected at 0 minutes, 5 minutes, and 10 minutes after induction in quadruplicate.

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Project description:The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi hfq deletion mutant to that of the wild type parental strain.

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Project description:The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi fis deletion mutant to that of the wild type parental strain.

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Haemophilus ducreyi 35000HPcpxA gene expression relative to 35000HP

Project description:Comparative analysis of the global gene expression of a Haemophilus ducreyi 35000HP cpxA deletion mutant relative to the wild type strain

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