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Genome wide trimethyl-H3K27 distibution in macrophages from wild-type and Jmjd3-/- macrophages.

ABSTRACT: Jmjd3 is trimethyl H3K27 specific demethylase required for M2 macrophage polarization. Genomic fragments obtained from wild-type and Jmjd3-/- mouse macrophages were immunoprecipitated with anti H3K27me3 Ab, and deep sequencing was performed. wild-type and Jmjd3-/- macrophages

ORGANISM(S): Mus musculus

SUBMITTER: Osamu Takeuchi

PROVIDER: E-GEOD-23297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection.

Satoh Takashi T Takeuchi Osamu O Vandenbon Alexis A Yasuda Koubun K Tanaka Yoshiaki Y Kumagai Yutaro Y Miyake Tohru T Matsushita Kazufumi K Okazaki Toshihiko T Saitoh Tatsuya T Honma Kiri K Matsuyama Toshifumi T Yui Katsuyuki K Tsujimura Tohru T Standley Daron M DM Nakanishi Kenji K Nakai Kenta K Akira Shizuo S

Nature immunology 20100822 10

Polarization of macrophages to M1 or M2 cells is important for mounting responses against bacterial and helminth infections, respectively. Jumonji domain containing-3 (Jmjd3), a histone 3 Lys27 (H3K27) demethylase, has been implicated in the activation of macrophages. Here we show that Jmjd3 is essential for M2 macrophage polarization in response to helminth infection and chitin, though Jmjd3 is dispensable for M1 responses. Furthermore, Jmjd3 (also known as Kdm6b) is essential for proper bone m ...[more]

PMID: 20729857

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