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Pmel CD8+ stim 1 vs. stim 2

ABSTRACT: Repeat experiments of CD8+ pmel effector cells following one and two rounds of restimulation

ORGANISM(S): Mus musculus

SUBMITTER: Deborah Surman

PROVIDER: E-GEOD-2348 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Acquisition of full effector function in vitro paradoxically impairs the in vivo antitumor efficacy of adoptively transferred CD8+ T cells.

Gattinoni Luca L Klebanoff Christopher A CA Palmer Douglas C DC Wrzesinski Claudia C Kerstann Keith K Yu Zhiya Z Finkelstein Steven E SE Theoret Marc R MR Rosenberg Steven A SA Restifo Nicholas P NP

The Journal of clinical investigation 20050601 6

T cell differentiation is a progressive process characterized by phenotypic and functional changes. By transferring tumor-specific CD8+ T cells into tumor-bearing mice at various stages of differentiation, we evaluated their efficacy for adoptive immunotherapy. We found that administration of naive and early effector T cells, in combination with active immunization and IL-2, resulted in the eradication of large, established tumors. Despite enhanced in vitro antitumor properties, more-differentia ...[more]

PMID: 15931392

Similar Datasets

Pmel CD8+ stim 1 vs. stim 2

Project description:Repeat experiments of CD8+ pmel effector cells following one and two rounds of restimulation Keywords: repeat sample

2005-03-02 | GSE2348 | GEO

Expression data for naÃ¯ve IL-2 and IL-12 primed Pmel-1 CD8+ T-cells

Project description:The expansion, trafficking and functional effectiveness of adoptively transferred CD8+ T-cells play a critical role in mediating effective anti-tumor immunity. However, the mechanisms which program the highly proliferative and functional state of CD8+ T-cells are not completely understood. We hypothesized that IL-12, a cytokine commonly induced by TLR activation, could enhance T-cell priming by altering responsiveness to antigen and cytokines. Priming of tumor specific CD8+ T-cells in the presence of IL-12 induced the acquisition of a 'polyfunctional' effector response and increased the generation of memory cells. Moreover, IL-12 priming also promoted high levels of the IL-2 receptor alpha-chain (CD25) and robust IL-2 mediated activation of STAT5. This sensitivity to IL-2 translated into enhanced in vivo proliferation of adoptively transferred CD8+ T-cells. Furthermore, real-time, in vivo imaging of T-cell trafficking confirmed the ability of IL-12 priming to drive in vivo proliferation. IL-12 priming enhanced the anti-tumor function of adoptively transferred cells by reducing established subcutaneous tumor burden, and significantly increasing survival in an established intracranial tumor model. Finally, IL-12 priming of human PBMCs generates tumor specific T-cells phenotypically and functionally similar to IL-12 primed Pmel-1 T-cells. These results highlight IL-12 as an important mediator of CD8+ T-cell effector function and anti-tumor immunity. We primed Pmel-1 TCR transgenic CD8+ T-cells with cognate antigen and either IL-2 or IL-12 and compared their gene expression profiles. This was used to identify pathways or genes necessary for anti-tumor activity in vivo. RNA was isolated from Pmel-1 T-cells primed with antigen and cytokine for 6 days and hybridized to Affymetrix arrays.

2010-10-19 | E-GEOD-22443 | biostudies-arrayexpress

Murine CD8+ Pmel Lymphocytes IL-2 vs. IL-15

Project description:Central memory tumor-reactive CD8+ T cells confer superior antitumor immunity compared to effector memory T cells

2010-06-10 | E-GEOD-2578 | biostudies-arrayexpress

Gene expression analysis in naïve and vaccine (peptide vs. DC) primed pmel-1 CD8 Tcells

Project description:We have shown that DC vaccine is superior to peptide vaccine in terms of priming and expansion of antigen-specific CD8+ T cells. DC vaccine-primed pmel-1 cells displayed better effecter functions than cells by peptide-primed cells in terms of cytokine production and externalization of cytotoxic granules. Furthermore DC vaccine-primed cells were metabolically distinct from peptide-primed cells. To confirm these findings, we performed a microarray analysis using splenic pmel-1 T cells from mice immunized with hgp100 peptide vaccine or DC vaccine. We also used splenic naïve pmel-1 T cells as a control.

2017-08-31 | GSE94538 | GEO

Murine CD8+ Pmel Lymphocytes IL-2 vs. IL-15

Project description:Central memory tumor-reactive CD8+ T cells confer superior antitumor immunity compared to effector memory T cells Keywords: other

2005-04-27 | GSE2578 | GEO

Real-time quantitative PCR analysis for miRNA in mouse CD8+ pMel-1 T cells

Project description:Mouse CD8+ pMel-1 transgenic T cells were primed by either splenic B cells or LPS-matured bone marrow-derived dendritic cells for 3 days in vitro. CD8+TCRb+ cells were then sorted for miRNA expression profiling by RT-qPCR.

2014-12-01 | GSE60884 | GEO

Whole transcriptome analysis of pmel-1 CD8+ T cells treated with rat IgG, 3H3, and 3H3 plus TKS-1antibodies

Project description:Mouse pmel-1 CD8+ T cells were treated with anti-4-1BB antibody, anti-4-1BBL antibody, or combination of both antibodies to see their effect on the T cells.

2023-08-01 | E-MTAB-13053 | biostudies-arrayexpress

The gene expression profiles of antigen-primed wildtype and Ddit3-/- CD8 Pmel T cells

Project description:T cell undergoes ER stress during regular priming which may lead to T cell dysfunction in cancer. ER stress downstream sensor Chop (encoded by Ddit3 gene) negatively regulates CD8 T cell function and cytotoxicity. Because Chop is a C/EBP homologous transcription factor, we sorted antigen-primed wildtype or ddit3-/- Pmel CD8 T cells perform RNA-seq and gene expression profile analysis.

2019-04-02 | GSE112823 | GEO

Expression data for naïve IL-2 and IL-12 primed Pmel-1 CD8+ T-cells

2010-10-19 | GSE22443 | GEO

Transcription profiling of mouse effector cells derived from naive or central memory pmel-1 CD8+ T cells

Project description:Effector cells for adoptive immunotherapy can be generated by in vitro stimulation of naÃ¯ve or memory subsets of CD8+ T cells. While the characteristics of CD8+ T cell subsets are well defined, the heritable influence of those populations on their effector cell progeny is not well understood. We studied effector cells generated from naÃ¯ve or central memory CD8+ T cells and found that they retained distinct gene expression signatures and developmental programs. Effector cells derived from central memory cells tended to retain their CD62L+ phenotype, but also to acquire KLRG1, an indicator of cellular senescence. In contrast, the effector cell progeny of naÃ¯ve cells displayed reduced terminal differentiation, and, following infusion, they displayed greater expansion, cytokine production, and tumor destruction. These data indicate that effector cells retain a gene expression imprint conferred by their naÃ¯ve or central memory progenitors, and they suggest a strategy for enhancing cancer immunotherapy. Experiment Overall Design: Effector cells were generated from naive or central memory CD8+ T cells. The cells were then rested (unstimulated) or restimulated (stimulated). This experimental design resulted in 4 groups (NaÃ¯ve-derived/stimulated, NaÃ¯ve-derived/unstimulated, Central memory-derived/stimulated, Central memory-derived/unstimulated). Three replicates from independent experiments were analyzed.

2009-06-23 | E-GEOD-16522 | biostudies-arrayexpress

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