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Expression data in Arabidopsis axe1-5 and met1-3 mutants.

ABSTRACT: We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis axe1-5 and met1-3 mutants. Fifteen-day-old Arabidopsis plants grown on the agar plates were harvested. The total RNA was prepared from 15-day-old Arabidopsis plants (axe1-5 (hda6 mutant), DR5 (parental line of axe1-5), met1-3 and Col) and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Motoaki Seki

PROVIDER: E-GEOD-23950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis HDA6 regulates locus-directed heterochromatin silencing in cooperation with MET1.

To Taiko Kim TK Kim Jong-Myong JM Matsui Akihiro A Kurihara Yukio Y Morosawa Taeko T Ishida Junko J Tanaka Maho M Endo Takaho T Kakutani Tetsuji T Toyoda Tetsuro T Kimura Hiroshi H Yokoyama Shigeyuki S Shinozaki Kazuo K Seki Motoaki M

PLoS genetics 20110428 4

Heterochromatin silencing is pivotal for genome stability in eukaryotes. In Arabidopsis, a plant-specific mechanism called RNA-directed DNA methylation (RdDM) is involved in heterochromatin silencing. Histone deacetylase HDA6 has been identified as a component of such machineries; however, its endogenous targets and the silencing mechanisms have not been analyzed globally. In this study, we investigated the silencing mechanism mediated by HDA6. Genome-wide transcript profiling revealed that the ...[more]

PMID: 21552333

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Expression data in Arabidopsis axe1-5 and met1-3 mutants.

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis axe1-5 and met1-3 mutants.

2011-05-16 | GSE23950 | GEO

Expression data in Arabidopsis hda6 mutant axe1-5 and its parental line DR5 under cold and control conditions.

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Expression data for the mom1-2 mutant of Arabidopsis

Project description:Heterochromatin is an inert region in the genome and composed of mainly remnants of transposons and repetitive elements. In Arabidopsis, the major heterchromatin regions are present at around centromeres (pericentromeric regions) and at a region on the short arm of chromosome 4 (heterochromatin knob). Histones and DNAs in heterochromatin have characteristic features with abundant H3H9me2 and cytosine methylation, respectively. Here, by using a genome tiling array, we showed that a subset of heterochromatin loci are silenced by the action of Morpheus' molecule 1 (MOM1) that is an epigeneic regulator for transcriptional gene silencing independent of global DNA and histone modification. Most of the up-regulated loci in the mom1 mutant carried sequences related to transposable elements but none of them was annotated as functional transposons. No specific subclass of transposons was targeted by MOM1 and loci that were unrelated to transposons but flanked by short tandem repeats were also shown to be under the control of MOM1. The results suggest the presence of an unknown level of regulatory network maintaining the silent state of heterochromain in the genome. Keywords: Epigenetic regulation of endogenous loci by Morpheus' Molecule 1 (MOM1) Three-week-old Arabidopsis plants (Col-0 and mom1-2) grown on soil were subjected to RNA extraction and the total RNA samples were used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

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Expression data in Arabidopsis hda6 mutant axe1-5 and its parental line DR5 under cold and control conditions.

2011-01-27 | GSE26873 | GEO

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2017-04-29 | GSE46365 | GEO

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