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Expression data in Arabidopsis fry1-6, xrn2-4, xrn3-3 and xrn4-6 mutants.

ABSTRACT: We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis fry1-6, xrn2-4, xrn3-3 and xrn4-6 mutants. The total RNA was prepared from 15-day-old Arabidopsis plants grown on the agar plates and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yukio Kurihara

PROVIDER: E-GEOD-32977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Surveillance of 3' Noncoding Transcripts Requires FIERY1 and XRN3 in Arabidopsis.

Kurihara Yukio Y Schmitz Robert J RJ Nery Joseph R JR Schultz Matthew D MD Okubo-Kurihara Emiko E Morosawa Taeko T Tanaka Maho M Toyoda Tetsuro T Seki Motoaki M Ecker Joseph R JR

G3 (Bethesda, Md.) 20120401 4

Eukaryotes possess several RNA surveillance mechanisms that prevent undesirable aberrant RNAs from accumulating. Arabidopsis XRN2, XRN3, and XRN4 are three orthologs of the yeast 5'-to-3' exoribonuclease, Rat1/Xrn2, that function in multiple RNA decay pathways. XRN activity is maintained by FIERY1 (FRY1), which converts the XRN inhibitor, adenosine 3', 5'-bisphosphate (PAP), into 5'AMP. To identify the roles of XRNs and FRY1 in suppression of non-coding RNAs, strand-specific genome-wide tiling a ...[more]

PMID: 22540040

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