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Microarray Analysis of differential gene expression in peripheral blood cells of patients with human essential hypertension

ABSTRACT: The polygenic nature of essential hypertension and its dependence on environmental factors pose a challenge for biomedical research. We hypothesized that microarray analysis of differential gene expression in peripheral blood cells would distinguish patients with hypertension from normotensive controls. We utilized microaaray analysis of differential gene expression in peripheral blood cells to identify differences in transcription profile of human essential hypertension compared with normotensive volunteers. Samples were pooled from participants who met with our recruitment criteria for RNA extraction and hybridization on Affymetrix Gene Chips Human Genome U133 Plus 2.0 Array. Experiments were performed at CapitalBio Corporation (Beijing, China)

ORGANISM(S): Homo sapiens

SUBMITTER: MELVIN KORKOR

PROVIDER: E-GEOD-24752 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of differential gene expression profile in peripheral blood cells of patients with human essential hypertension.

Korkor Melvin T MT Meng Fan Bo FB Xing Shen Yang SY Zhang Mu Chun MC Guo Jin Rui JR Zhu Xiao Xue XX Yang Ping P

International journal of medical sciences 20110227 2

The polygenic nature of essential hypertension and its dependence on environmental factors pose a challenge for biomedical research. We hypothesized that the analysis of gene expression profiles from peripheral blood cells would distinguish patients with hypertension from normotensives. In order to test this, total RNA from peripheral blood cells was isolated. RNA was reversed-transcribed and labeled and gene expression analyzed using significance Analysis Microarrays (Stanford University, CA, U ...[more]

PMID: 21369372

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