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Gene expression in response to genetic and chemical perturbations of chromatin structure

ABSTRACT: Microarray expression profiling was used to identify genes expressed misexpressed in wild-type Arabidopsis seedlings treated with 5-aza-2’ deoxyctidine (5AC) or trichostatin A (TSA), and in decrease in dna methylation1 (ddm1) mutant seedlings. 10 day-old Arabidopsis seedlings (ecotype Ws) were grown in 0.5X MS media (containing 1% sucrose) at 21 C in continuous white light. Media (including 5AC and TSA) were refreshed after 5 days of growth. RNA was extracted using the Qiagen Plant RNeasy kit. Three biological replicates for each treatment and genotype were used.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Karen Hudson

PROVIDER: E-GEOD-25067 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Changes in global gene expression in response to chemical and genetic perturbation of chromatin structure.

Hudson Karen K Luo Song S Hagemann Nicole N Preuss Daphne D

PloS one 20110603 6

DNA methylation is important for controlling gene expression in all eukaryotes. Microarray analysis of mutant and chemically-treated Arabidopsis thaliana seedlings with reduced DNA methylation revealed an altered gene expression profile after treatment with the DNA methylation inhibitor 5-aza-2' deoxycytidine (5-AC), which included the upregulation of expression of many transposable elements. DNA damage-response genes were also coordinately upregulated by 5-AC treatment. In the ddm1 mutant, more ...[more]

PMID: 21673996

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Gene expression in response to genetic and chemical perturbations of chromatin structure

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2011-06-03 | GSE25067 | GEO

Interactions between reactive oxygen species stress and brassinosteroid hormone signaling in Arabidopsis seedlings

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2011-02-28 | E-GEOD-20756 | biostudies-arrayexpress

Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein

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2010-04-12 | E-GEOD-13881 | biostudies-arrayexpress

Auxin stimulates brassinosteroid biosynthesis in Arabidopsis roots

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2011-02-15 | E-GEOD-12964 | biostudies-arrayexpress

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2013-06-30 | E-GEOD-37484 | biostudies-arrayexpress

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2010-12-31 | E-MEXP-2270 | biostudies-arrayexpress

Expression analysis of Arabidopsis seedlings 3h and 2h after transfer to sucrose

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Transcription profiling of Arabidopsis after deuterium oxide treatment

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