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Gene expression profile of calcium mediated prevention of colorectal cancer in a mouse model

ABSTRACT: To further elucidate the mechanism of calcium mediated chemoprevention of colorectal cancer,we have employed whole genome microarray expression profiling as a discovery platform to identify genes that differentially expressed in mice from high calcium group compared those from DMH group.The mice of DMH group were received normal diet (calcium concentration,1.24%) and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks,the mice of high calcium group were received high calcium diet(calcium concentration,3%) and subcutaneous injection of 1,2-Dimethylhydrazine at a dose of 20 mg/kg once weekly for 20 weeks.The mice were sarcrificed at 24 week.Nine normal colonic mucosa (3 from DMH group, 3 from high calcium group mice with tumors, and 3 from high calcium group mice without tumors) were used for the microarray analysis. The differentially expressed genes were indentified between high calcium group and DMH group,as long as differentially expressed pathways and gene ontology terms.

ORGANISM(S): Mus musculus

SUBMITTER: Jing-Yuan Fang

PROVIDER: E-GEOD-25680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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