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Transcription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs

ABSTRACT: HUVEC were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparsion of the gene profiles revealed TNF-mediated gene expression changes in HUVEC.

ORGANISM(S): Homo sapiens

SUBMITTER: Dorothee Viemann

PROVIDER: E-GEOD-2639 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TNF induces distinct gene expression programs in microvascular and macrovascular human endothelial cells.

Viemann Dorothee D Goebeler Matthias M Schmid Sybille S Nordhues Ursula U Klimmek Kerstin K Sorg Clemens C Roth Johannes J

Journal of leukocyte biology 20060414 1

The relevance of the diversity of endothelial cells (ECs) for the response to inflammatory stimuli is currently not well defined. Using oligonucleotide microarray technique, we systematically analyzed the tumor necrosis factor (TNF)-induced expression profile in human microvascular ECs (HMEC) and macrovascular human umbilical vein ECs (HUVEC), analyzing 13,000 human genes by microarray analysis. Using strict inclusion and exclusion criteria, microarray analysis revealed that about half of the TN ...[more]

PMID: 16617158

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