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ChIP-seq of tumour associated macrophages derived from high grade ovarian serous carcinoma patient ascites

ABSTRACT: Tumor associated macrophages of one patient were assayed for histone modifications, methylation (MIRA) and CEBP.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Homo sapiens

SUBMITTER: Till Adhikary

PROVIDER: E-MTAB-6297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin Binding of c-REL and p65 Is Not Limiting for Macrophage IL12B Transcription During Immediate Suppression by Ovarian Carcinoma Ascites.

Unger Annika A Finkernagel Florian F Hoffmann Nathalie N Neuhaus Felix F Joos Barbara B Nist Andrea A Stiewe Thorsten T Visekruna Alexander A Wagner Uwe U Reinartz Silke S Müller-Brüsselbach Sabine S Müller Rolf R Adhikary Till T

Frontiers in immunology 20180627

Tumors frequently exploit homeostatic mechanisms that suppress expression of IL-12, a central mediator of inflammatory and anti-tumor responses. The p40 subunit of the IL-12 heterodimer, encoded by IL12B, is limiting for these functions. Ovarian carcinoma patients frequently produce ascites which exerts immunosuppression by means of soluble factors. The NFκB pathway is necessary for transcription of IL12B, which is not expressed in macrophages freshly isolated from ascites. This ra ...[more]

PMID: 29997615

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