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Differential effects of inhibition of bone morphogenic protein (BMP) signalling on T-cell activation and differentiation

ABSTRACT: Dorsomorphin is a small molecule inhibitor of type I bone morphogenic protein receptors (BMPRs). We have found that dorsomorphin affects a wide range of T cell function. In order to obtain the bigger picture of the effects of DM in T cell activation. transcriptomic analysis was performed using mouse primary CD25-CD4+ T cells with either DM (4 μM) or vehicle in the presence or absence of stimulation by anti-CD3 and -CD28 antibodies. Mouse CD4+ T cells were prepared and cultured with or without stimulation by plate bound anti-CD3 mAb and soluble anti-CD28 mAb for 1 hour. Subsequently, 4 μM DM or DMSO was added and cells were incubated for 6 hours. Total RNA was isolated using Qiagen RNeasy mini kits.

ORGANISM(S): Mus musculus

SUBMITTER: Masahiro Ono 

PROVIDER: E-GEOD-27378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential effects of inhibition of bone morphogenic protein (BMP) signalling on T-cell activation and differentiation.

Yoshioka Yumiko Y   Ono Masahiro M   Osaki Motonao M   Konishi Ikuo I   Sakaguchi Shimon S  

European journal of immunology 20111220 3

Bone morphogenetic proteins (BMPs) are involved in patterning and cellular fate in various organs including the thymus. However, the redundancy of BMPs and their receptors have made it difficult to analyse their physiological roles. Here, we investigated the role of BMP signalling in peripheral CD4(+) T cells by analysing the effects of an inhibitor of BMP signalling, dorsomorphin. Dorsomorphin suppressed phosphorylation of SMAD1/5/8, suggesting that BMP signalling naturally occurs in T cells.  ...[more]

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