Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of 5-amino-4-imidazolecarboxamide ribotide (AICAr) supplementation on global transcription

ABSTRACT: We investigated the effects of AICAr supplementation on the global transcriptome of Y2950. The Y2950 strain was grown with or without AICAr. For each comparison, we made 2 biological replicates, swapping dyes.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: sophie lemoine

PROVIDER: E-GEOD-29019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Physiological and toxic effects of purine intermediate 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) in yeast.

Hürlimann Hans C HC Laloo Benoît B Simon-Kayser Barbara B Saint-Marc Christelle C Coulpier Fanny F Lemoine Sophie S Daignan-Fornier Bertrand B Pinson Benoît B

The Journal of biological chemistry 20110712 35

5-Amino-4-imidazolecarboxamide ribonucleotide 5'-phosphate (AICAR) is a monophosphate metabolic intermediate of the de novo purine synthesis pathway that has highly promising metabolic and antiproliferative properties. Yeast mutants unable to metabolize AICAR are auxotroph for histidine. A screening for suppressors of this phenotype identified recessive and dominant mutants that result in lowering the intracellular AICAR concentration. The recessive mutants affect the adenosine kinase, which is ...[more]

PMID: 21757731

Similar Datasets

Role of Ado1p in the transcriptional response associated with AICAr supplementation

Project description:We investigated the role of Ado1p associated with AICAr supplementation on the global transcriptome of Y3188. The Y3188 strain was grown with or without AICAr. For each comparison, we made 2 biological replicates, swapping dyes.

2011-08-31 | E-GEOD-29020 | biostudies-arrayexpress

Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain. The ade3 mutant strain (Y4143) was compared to the WT strain (Y2946). For each comparison, we made 2 technical replicates, swapping dyes.

2011-08-31 | E-GEOD-29021 | biostudies-arrayexpress

Looking for mitochondrial associated RNA

Project description:We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNAs between free and mitochondrion-bound polysomes. Keywords: Mitochondrial-associated RNA localization in cells GSM239122-GSM239127: mitochondrial associated RNA (three biological replicates each in dye swap) GSM239128-GSM239131: mitochondrial associated RNA in presence of 200µg/ml cycloheximide (two biological replicates each in dye swap) GSM239132-GSM239135: mitochondrial associated RNA in presence of 2.1mM puromycin (two biological replicates each in dye swap) GSM239136-GSM239139: mitochondrial associated RNA in Delta Puf3 strain (two biological replicates each in dye swap)

2008-06-01 | E-GEOD-9393 | biostudies-arrayexpress

Effect of adk1 knock-out mutation on global transcription

Project description:We investigated the effects of the variation in intracellular adenylic nucleotides pools due to the absence of the adenylate kinase Adk1p on the global yeast transcriptome. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription on total RNA from cells containing or not Adk1p. One biological sample in dye swap

2008-04-17 | E-GEOD-9553 | biostudies-arrayexpress

Ploidy influences the cellular responses of gross chromosomal rearrangements in Saccharomyces cerevisiae

Project description:We investigated the effects of the ploidy on cellular response in strains carrying various types of gross chromosomal rearrangements. Fourteen mutated strains (6 haploid strains and 8 diploid strains) were compared to their associated parental strain (haploid or diploid parental strain). For each comparison, 2 microarray experiments implying biological replicates were performed.

2011-06-17 | E-GEOD-28285 | biostudies-arrayexpress

Tye7 regulates yeast Ty1 retrotransposon sense and antisense transcription in response to adenylic nucleotides stress

Project description:We investigated the effects of adenine starvation on the global yeast transcriptome. In this work, global transcription analyses were combined with measurements of intracellular nucleotides pools by HPLC. The bas1delta strain grown with or without adenine was compared to the WT strain grown with or without adenine. For each comparison, we performed 2 technical replicates, swapping dyes.

2012-02-10 | E-GEOD-28579 | biostudies-arrayexpress

Role of the AMP deaminase Amd1p in purines interconversion in Saccharomyces cerevisiae

Project description:In this work, we show that under specific conditions, defect in AMP deaminase activity strongly impairs yeast cell growth and we establish that AMP deaminase is required for correct balance between adenylic and guanylic nucleotides. Study of the Amd1p homologs, Yjl070p and Ybr284p, reveals that overexpression of these proteins cannot suppress phenotypes associated to /amd1/ deletion but instead mimic in a wild-type strain a loss of Amd1p function. Data presented combined global transcription analyses to measurements of intracellular nucleotides pools by HPLC. Keywords: yeast strains implied in purines interconversion. 4 biological samples in dye-swap.

2010-06-20 | E-GEOD-9557 | biostudies-arrayexpress

Silicon metabolism in diatoms

Project description:We investigated the effects of extracellular silicic acid on the whole Phaeodactylum triconutum transcriptome. In this work, global transcription analyses were combined with comparative studies of other elements, and detailed analyses of the silicon transporters. Our results provide insight on Si-sensing genes and further extend current models for Si uptake. Keywords: Comparative transcriptome analyses from Phaeodactylum triconutum fusiform cells acclimated in a Si-free or silicic acid medium. Three biological samples in dye swap

2009-09-29 | E-GEOD-12015 | biostudies-arrayexpress

Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors

Project description:This SuperSeries is composed of the following subset Series: GSE9603: Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors on NIA 15k microarray GSE9619: Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors on NeuroDev microarray Keywords: SuperSeries Refer to individual Series

2010-05-26 | E-GEOD-9620 | biostudies-arrayexpress

Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors on NeuroDev microarray

Project description:Branchiomotor neurons are an important class of cranial motor neurons that innervate the branchial-arch-derived muscles of the face, jaw and neck. They arise in the ventralmost progenitor domain of the rhombencephalon characterized by expression of the homeodomain transcription factors Nkx2.2 and Phox2b. Phox2b in particular plays a key role in the specification of branchiomotor neurons. In its absence, generic neuronal differentiation is defective in the progenitor domain, and no branchiomotor neurons are produced. Conversely, ectopic expression of Phox2b in spinal regions of the neural tube promotes cell cycle exit and neuronal differentiation and at the same time induces genes and an axonal phenotype characteristic for branchiomotor neurons. How Phox2b exerts its pleiotropic functions, both as a proneural gene and a neuronal subtype determinant, has remained unknown. To gain further insight into the genetic programme downstream of Phox2b we searched for novel Phox2b-regulated genes by cDNA microarray (here NeuroDev slides) analysis of facial branchiomotor neuron precursors from heterozygous and homozygous Phox2b mutant embryos. Keywords: Phox2b-regulated genes identification Four biological replicates each in dye swap

2010-05-25 | E-GEOD-9619 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data