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Effect of 5-amino-4-imidazolecarboxamide ribotide (AICAr) supplementation on global transcription

ABSTRACT: We investigated the effects of AICAr supplementation on the global transcriptome of Y2950. The Y2950 strain was grown with or without AICAr. For each comparison, we made 2 biological replicates, swapping dyes.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: sophie lemoine

PROVIDER: E-GEOD-29019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Physiological and toxic effects of purine intermediate 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) in yeast.

Hürlimann Hans C HC Laloo Benoît B Simon-Kayser Barbara B Saint-Marc Christelle C Coulpier Fanny F Lemoine Sophie S Daignan-Fornier Bertrand B Pinson Benoît B

The Journal of biological chemistry 20110712 35

5-Amino-4-imidazolecarboxamide ribonucleotide 5'-phosphate (AICAR) is a monophosphate metabolic intermediate of the de novo purine synthesis pathway that has highly promising metabolic and antiproliferative properties. Yeast mutants unable to metabolize AICAR are auxotroph for histidine. A screening for suppressors of this phenotype identified recessive and dominant mutants that result in lowering the intracellular AICAR concentration. The recessive mutants affect the adenosine kinase, which is ...[more]

PMID: 21757731

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