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Effect of adk1 knock-out mutation on global transcription

ABSTRACT: We investigated the effects of the variation in intracellular adenylic nucleotides pools due to the absence of the adenylate kinase Adk1p on the global yeast transcriptome. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription on total RNA from cells containing or not Adk1p. One biological sample in dye swap

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Laurent Jourdren

PROVIDER: E-GEOD-9553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Co-regulation of yeast purine and phosphate pathways in response to adenylic nucleotide variations.

Gauthier Sébastien S Coulpier Fanny F Jourdren Laurent L Merle Michel M Beck Stefanie S Konrad Manfred M Daignan-Fornier Bertrand B Pinson Benoît B

Molecular microbiology 20080421 6

Adenylate kinase (Adk1p) is a pivotal enzyme in both energetic and adenylic nucleotide metabolisms. In this paper, using a transcriptomic analysis, we show that the lack of Adk1p strongly induced expression of the PHO and ADE genes involved in phosphate utilization and AMP de novo biosynthesis respectively. Isolation and characterization of adk1 point mutants affecting PHO5 expression revealed that all these mutations also severely affected Adk1p catalytic activity, as well as PHO84 and ADE1 tra ...[more]

PMID: 18433446

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