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Role of the AMP deaminase Amd1p in purines interconversion in Saccharomyces cerevisiae

ABSTRACT: In this work, we show that under specific conditions, defect in AMP deaminase activity strongly impairs yeast cell growth and we establish that AMP deaminase is required for correct balance between adenylic and guanylic nucleotides. Study of the Amd1p homologs, Yjl070p and Ybr284p, reveals that overexpression of these proteins cannot suppress phenotypes associated to /amd1/ deletion but instead mimic in a wild-type strain a loss of Amd1p function. Data presented combined global transcription analyses to measurements of intracellular nucleotides pools by HPLC. Keywords: yeast strains implied in purines interconversion. 4 biological samples in dye-swap.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Olesia Lisova

PROVIDER: E-GEOD-9557 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Phenotypic consequences of purine nucleotide imbalance in Saccharomyces cerevisiae.

Saint-Marc Christelle C Pinson Benoît B Coulpier Fanny F Jourdren Laurent L Lisova Olesia O Daignan-Fornier Bertrand B

Genetics 20090727 2

Coordinating homeostasis of multiple metabolites is a major task for living organisms, and complex interconversion pathways contribute to achieving the proper balance of metabolites. AMP deaminase (AMPD) is such an interconversion enzyme that allows IMP synthesis from AMP. In this article, we show that, under specific conditions, lack of AMPD activity impairs growth. Under these conditions, we found that the intracellular guanylic nucleotide pool was severely affected. In vivo studies of two AMP ...[more]

PMID: 19635936

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