Technical and biological variance structure in mRNA-Seq data
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Brian Bot
PROVIDER: E-GEOD-29022 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Technical and biological variance structure in mRNA-Seq data: life in the real world.
Oberg Ann L AL Bot Brian M BM Grill Diane E DE Poland Gregory A GA Therneau Terry M TM
BMC genomics 20120707
<h4>Background</h4>mRNA expression data from next generation sequencing platforms is obtained in the form of counts per gene or exon. Counts have classically been assumed to follow a Poisson distribution in which the variance is equal to the mean. The Negative Binomial distribution which allows for over-dispersion, i.e., for the variance to be greater than the mean, is commonly used to model count data as well.<h4>Results</h4>In mRNA-Seq data from 25 subjects, we found technical variation to gen ...[more]
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