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Implication of Nos2 inactivation on the transcriptome of developing cerebellum and Ptch1+/- medulloblastomas (mRNA)

ABSTRACT: The Ptch1+/- strain constitues an established mouse model for the Shh-driven type of medulloblastoma. Combined Ptch1+/- Nos2-/- mice show a two-fold increased incidence for this tumor. Here, the impact of Nos2 inactivation on medulloblastoma development was investigated by gene expression profiling of tumor samples as well as healthy cerebellum at different ages and genotypes. Medulloblastoma samples from three Ptch1+/- and six combined Ptch1+/- Nos2-/- mice were analyzed. Healthy cerebellum taken from mice at postnatal day nine, six weeks after birth, and about 1 year of age were analyzed for wildtype animals and the genotypes Ptch1+/-, Ptch1+/- Nos2-/-, and Nos2-/-. The cerebellar developmental stages at six weeks and one year were measured in three biological replicates, while samples taken at postnatal day six consisted of pooled individual specimen. These were measured in three replicates being amplified and labelled in separate reactions. All samples were subjected to two-color hybridizations against Universal Reference RNA (Stratagene) with color-switch experiments yielding two technical replicates, respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Daniel Haag

PROVIDER: E-GEOD-29192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ptch1+/- Nos2-/- medulloblastomas

Project description:This SuperSeries is composed of the following subset Series: GSE29192: Implication of Nos2 inactivation on the transcriptome of developing cerebellum and Ptch1+/- medulloblastomas (mRNA) GSE29199: Implication of Nos2 inactivation on genomic changes in Ptch1+/- medulloblastomas (array-CGH) Refer to individual Series

2011-12-01 | E-GEOD-29201 | biostudies-arrayexpress

Implication of Nos2 inactivation on genomic changes in Ptch1+/- medulloblastomas (array-CGH)

Project description:The Ptch1+/- strain constitues an established mouse model for the Shh-driven type of medulloblastoma. Combined Ptch1+/- Nos2-/- mice show a two-fold increased incidence for this tumor. Here, the impact of Nos2 inactivation on copy number alterations during medulloblastoma development was investigated by array-based comparative genomic hybridization (arrayCGH) of tumor samples from both genotypes. Medulloblastoma samples from five Ptch1+/- and seven compound Ptch1+/- Nos2-/- mice were analyzed. Cy5-labeled tumor DNA was combined with corresponding Cy3-labeled reference healthy wildtype genomic DNA to receive either sex-matched sample pairs or pairs of different gender for internal negative or positive control.

2011-12-01 | E-GEOD-29199 | biostudies-arrayexpress

Project description:Ptch1+/- Nos2-/- medulloblastomas

| PRJNA140045 | ENA

Transcription profiling of human nevoid basal cell carcinoma syndrome primary fibroblasts with non-sense or miss-sense mutations in the tumor supressor gene PTCH1

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2009-03-19 | E-TABM-549 | biostudies-arrayexpress

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Project description:Microarray technology was used to identify new predictive biomarkers in samples of clinically homogeneus patients.

2008-10-26 | E-TABM-364 | biostudies-arrayexpress

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2004-07-15 | E-MEXP-68 | biostudies-arrayexpress

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2004-07-01 | E-MEXP-112 | biostudies-arrayexpress

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2005-06-03 | E-WMIT-4 | biostudies-arrayexpress

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