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Gene expression profiles of effector T cells induced by mTOR inhibition

ABSTRACT: To further develop our gene expression approach to treatment of autoimmunity, we have employed whole genome microarray expression profiling as a discovery platform to identify genes differentially expressed upon immunosuppressor treatment responsible for inhibition of T cell effector functions. Human CD4+CD25- cells from healthy donors were pretreated or not with rapamycin and stimulated with anti-CD3 and anti-CD28 for 12h. mTOR (Mammalian Target of Rapamycin) inhibition-induced gene expression was evaluated in human effector T cells after 1 hour rapamycin pretreatment and compared to vehicle-pretreated cells. Three independent experiments were performed for each of the two different experimental conditions using different donors for each experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Dario Greco 

PROVIDER: E-GEOD-29606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses.

Procaccini Claudio C   De Rosa Veronica V   Galgani Mario M   Carbone Fortunata F   Cassano Silvana S   Greco Dario D   Qian Kui K   Auvinen Petri P   Calì Gaetano G   Stallone Giovanni G   Formisano Luigi L   La Cava Antonio A   Matarese Giuseppe G  

Journal of immunology (Baltimore, Md. : 1950) 20120817 6

The sensing by T cells of metabolic and energetic changes in the microenvironment can determine the differentiation, maturation, and activation of these cells. Although it is known that mammalian target of rapamycin (mTOR) gauges nutritonal and energetic signals in the extracellular milieu, it is not known how mTOR and metabolism influence CD4+CD25-FOXP3- effector T cell (Teff) responses. In this article, we show that leptin-induced activation of mTOR, which, in turn, controls leptin production  ...[more]

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