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Gene expression profile of Tet1 knockout mouse embryonic stem cells (mESCs)

ABSTRACT: Global gene expression profile of Tet1 knockout ES cells is compared to wild-type ES cells. All ES lines used are V6.5 (mix 129 C57BL6 backgound). 2 Tet1 KO mice compared to 1 Tet1 wild type mouse.

ORGANISM(S): Mus musculus

SUBMITTER: Albert Cheng

PROVIDER: E-GEOD-30748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development.

Dawlaty Meelad M MM Ganz Kibibi K Powell Benjamin E BE Hu Yueh-Chiang YC Markoulaki Styliani S Cheng Albert W AW Gao Qing Q Kim Jongpil J Choi Sang-Woon SW Page David C DC Jaenisch Rudolf R

Cell stem cell 20110801 2

The Tet family of enzymes (Tet1/2/3) converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Mouse embryonic stem cells (mESCs) highly express Tet1 and have an elevated level of 5hmC. Tet1 has been implicated in ESC maintenance and lineage specification in vitro but its precise function in development is not well defined. To establish the role of Tet1 in pluripotency and development, we have generated Tet1 mutant mESCs and mice. Tet1(-/-) ESCs have reduced levels of 5hmC and subtle ch ...[more]

PMID: 21816367

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