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Integrated epigenome profiling of repressive histone modifications, DNA methylation and gene expression in normal and malignant urothelial cells [ChIP-Seq data]

ABSTRACT: We profiled two repressive histone modifications (H3K9m3 and H3K27m3) using ChIP-Seq in normal urothelial cells and cell lines representing non-invasive and invasive tumors. Two bladder cancer cell lines (RT112 and EJ/T24 from ATCC) and normal human urothelial (NHU) cells were treated according to standard ChIP-Seq protocols using antibodies against the histone modifications H3K9m3 and H3K27m3 (from Millipore). The sequencing reads were mapped against the human genome (GRCh37/hg19).

ORGANISM(S): Homo sapiens

SUBMITTER: Andreas Gogol-DM-CM-6ring

PROVIDER: E-GEOD-31125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrated epigenome profiling of repressive histone modifications, DNA methylation and gene expression in normal and malignant urothelial cells.

Dudziec Ewa E Gogol-Döring Andreas A Cookson Victoria V Chen Wei W Catto James J

PloS one 20120307 3

Epigenetic regulation of gene expression is commonly altered in human cancer. We have observed alterations of DNA methylation and microRNA expression that reflect the biology of bladder cancer. This common disease arises by distinct pathways with low and high-grade differentiation. We hypothesized that epigenetic gene regulation reflects an interaction between histone and DNA modifications, and differences between normal and malignant urothelial cells represent carcinogenic events within bladder ...[more]

PMID: 22412920

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Epigenome profiling of repressive histone modifications, DNA methylation and gene expression in normal and malignant urothelial cells

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2011-09-19 | E-GEOD-31864 | biostudies-arrayexpress

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Epigenome profiling of repressive histone modifications, DNA methylation and gene expression in normal and malignant urothelial cells

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